Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 243305
View all Marapsin/Pancreasin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Marapsin/Pancreasin
Ala23-Lys290
Accession # Q9BQR3

Specificity

Detects human Marapsin/Pancreasin in direct ELISAs and Western blots. Reacts with an epitope within the mature chain. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Marapsin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Marapsin/Pancreasin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Marapsin/Pancreasin

Marapsin, pancreasin, and channel-activating protease 2 (CAP-2) are different names for the same serine protease that is expressed strongly in the pancreas (1, 2). The protein is synthesized with a signal peptide (amino acids 1‑22), a pro peptide (aa 23‑34) and a mature chain (aa 35‑290) corresponding to the serine protease domain.

References

  1. Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
  2. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Alternate Names

CAP-2, MPN, Pancreasin, PRSS27

Entrez Gene IDs

83886 (Human); 213171 (Mouse)

Gene Symbol

PRSS27

UniProt

Q9BQR3

Additional Marapsin/Pancreasin Products

Product Documents for Human Marapsin/Pancreasin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Marapsin/Pancreasin Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

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