Detects human MCAM/CD146 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ALCAM, recombinant mouse (rm) MAdCAM-1, and rmOCAM is observed and less than 1% cross-reactivity with rhBCAM, rhEpCAM, rhNCAM-L1, and rhTROP-2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146 Met1-Gly559 Accession # AAA20824
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human MCAM/CD146
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MCAM/CD146 in Human Melanoma. MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MCAM, also known as MUC18, is an Ig-superfamily glycoprotein that shows significant homology to several cell adhesion molecules, notably N-CAM. It was identified as an antigen on metastatic melanoma cells and is also expressed on a variety of normal and transformed cell types. MCAM expression is associated with tumor progression and metastasis, and may be involved in embryonic neuronal development.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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