Detects human MCAM/CD146 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ALCAM, recombinant mouse (rm) MAdCAM-1, and rmOCAM is observed and less than 1% cross-reactivity with rhBCAM, rhEpCAM, rhNCAM-L1, and rhTROP-2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146 Met1-Gly559 Accession # AAA20824
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human MCAM/CD146
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MCAM/CD146 in Human Melanoma. MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MCAM, also known as MUC18, is an Ig-superfamily glycoprotein that shows significant homology to several cell adhesion molecules, notably N-CAM. It was identified as an antigen on metastatic melanoma cells and is also expressed on a variety of normal and transformed cell types. MCAM expression is associated with tumor progression and metastasis, and may be involved in embryonic neuronal development.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.