Human MD-2 Antibody

Catalog # Availability Size / Price Qty
AF1787
AF1787-SP
Product Details
Citations (1)
FAQs
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Human MD-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MD-2 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant human MD-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MD-2
Glu17-Asn160
Accession # NP_001123946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MD‑2 (Catalog # 1787-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MD2

MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that shares conserved cysteine residues and significant sequence similarity (23%) with MD-1. The gene of human MD-2 encodes a 160 amino acid residue (aa) precursor protein with a 16 aa signal peptide and a 144 aa mature protein, which contains 2 N-glycosylation sites (1). Recombinant secreted MD-2 has been found to exist as disulfide-linked dimers and oligomers (2).

Both MD-1 and MD-2 are accessory molecules that associate with the extracellular leucine-rich repeats (LRR) of Toll-like receptor (TLR) family members, which are type I transmembrane receptors that regulate innate immune responses to microbial pathogens (3, 4). MD-1 binds to RP105 on B cells and macrophages to form the signaling receptor complex for lipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria. Similarly, MD-2 interacts with TLR-4 to form the heteromeric receptor that confers LPS responsiveness. MD-2 also associates with TLR-2, albeit with less avidity, to confer responsiveness to cell wall components from both Gram-positive and Gram-negative bacteria. MD-1 and MD-2 are also required for the correct targeting of the TLRs to the cell surface. Although MD-2 glycosylation is not crucial for its surface expression and interaction with TLR-4, it is required for LPS binding and signaling (5).

References
  1. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  2. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
  3. Nagai, Y. et al. (2002) Nature Immunology 3:667.
  4. Akashi, S. et al. (2003) J. Exp. Med. 198:1035.
  5. Correia, J. and R. Ulevitch (2002) J. Biol. Chem. 277:1845.
Long Name
Myeloid Differentiation Protein 2/Lymphocyte Antigen 96
Entrez Gene IDs
23643 (Human); 17087 (Mouse); 102118398 (Cynomolgus Monkey)
Alternate Names
ESOP1; ESOP-1; LY96; ly-96; lymphocyte antigen 96; MD2; MD-2; myeloid differentiation protein-2; Protein MD-2

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Citation for Human MD-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis.
    Authors: Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, Mantovani A, Moretti S, Oikonomous V, De Santis R, Carvalho A, Salvatori G, Romani L
    J Immunol, 2014;193(5):2340-8.
    Species: Human
    Sample Types: Protein
    Applications: Functional Assay

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