Human MDGA1 Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Human MDGA1 Antibody Summary

Species Reactivity
Detects human MDGA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human MDGA2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human MDGA1
Accession # Q8NFP4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human MDGA1 (Catalog # 5055-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1; also named GPIM) is a 140 kDa member of the Ig superfamily of proteins (1). Human MDGA1 is synthesized as a 955 amino acid (aa) precursor that contains an 18 aa signal sequence, a 914 aa mature chain, and a 23 aa propeptide. The mature chain consists of six Ig-like domains, a fibronectin type III domain (aa 640‑739), a MAM domain (aa 751‑918), 11 potential sites for N-linked glycosylation, and a GPI anchor (aa 932) (1‑3). Alternative splicing in human MDGA1 produces an isoform variant that shows a 48 aa substitution for aa corresponding to positions 926‑955. Mature human MDGA1 shares 96%, 94%, and 80% aa sequence identity with mature rat, mouse, and chick MDGA1, respectively. MDGA1 is structurally similar to other IgCAMS, such as the L1 family and axonin 1, which have roles in cell adhesion, migration, and process outgrowth (4). Expression analysis in normal human tissues reveals that MDGA1 is expressed as a 5 kb and 9.5 kb mRNA (2). The smaller transcript is highly expressed in some human cancer cell lines, as well as in different primary tumors (lung, colon, uterus, stomach, and breast) (2). Gene expression is higher in several tumor tissues as compared with corresponding normal tissues, and may be involved in tumor progression (2). The 9.5 kb transcript is found exclusively in the central and peripheral nervous system (1‑2, 4). One study shows that MGDA1 is expressed in mice neocortex by layer 2/3 neurons throughout their development (4), and it is involved in controlling the migration and lamination of layer 2/3 neurons (5).

  1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
  2. De Juan, C. et al. (2002) Oncogene 21:3089.
  3. Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
  4. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
  5. Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
Long Name
MAM Domain Containing Glycosylphosphatidylinositol Anchor 1
Entrez Gene IDs
266727 (Human); 74762 (Mouse)
Alternate Names
FLJ45018; GPIM; MAM domain containing glycosylphosphatidylinositol anchor 1; MAMDC3; MDGA1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human MDGA1 Antibody

There are currently no reviews for this product. Be the first to review Human MDGA1 Antibody and earn rewards!

Have you used Human MDGA1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review