hh Human Melan-A/MART-1 Antibody AF8008: R&D Systems

Human Melan-A/MART-1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Melan-A/MART-1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Melan-A/MART-1
    Asn52-Pro118
    Accession # Q16655
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Melan-A/MART-1 by Western Blot. Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Melan-A/
MART-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Melan-A/MART-1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Melan-A/MART-1 by Simple WesternTM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Melan-A/MART-1 at approximately 21 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Melan-A/MART-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8008) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Melan-A/MART-1
Melan-A (MeLANoma-A; also MART-1, LB39-AA and SK29-AA, MLANA) is a 18-24 kDa member of a melanocyte lineage-specific, generally structurally-unrelated family of proteins. It is expressed only in melanocytes, retinal pigment epithelium and melanoma cells. Melan-A is involved in melanosome formation, and appears to stabilize both GPCR143/OA-1 and PMEL, thus ensuring the formation of a stage 2 (melanin production within a fibrillar matrix) melanosome. It is typically found in the Golgi, and upon inversion of its topology, becomes embedded in the ER. It may also appear in endosomes and on the cell surface where, in theory, it is recognizable by a special naïve type of CD8+ T cell that is derived from the thymus. Notably, these cells normally appear to be unresponsive, even in the face of exposure to skin melanocytes during wound healing. Human MLANA is a 118 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 26 aa extracellular region (aa 1-26), and a 71 aa cytoplasmic domain (aa 48-118). There is one utilized phosphorylation site at Ser108, and the molecule is known to undergo acylation, creating a MW of 23-34 kDa in SDS-PAGE. Over aa 52-118, human Melan-A shares 62% aa sequence identity with mouse MLANA.
  • Entrez Gene IDs:
    2315 (Human); 77836 (Mouse); 293890 (Rat)
  • Alternate Names:
    Antigen LB39-AA; Antigen SK29-AA; Mart 1 Melan A; MART-1; MART1MART-1; MelanA; Melan-A; melanoma antigen recognized by T-cells 1; MLANA; Protein Melan-A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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