Human MELK Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MELK in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MELK
    Gln341-Ala470
    Accession # Q14680
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MELK by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line, MO7e human megakaryocytic leukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MELK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MELK in Human Breast Cancer Tissue. MELK was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MELK

MELK (maternal embryonic leucine zipper kinase; also HPK38) is a member of the Snf1/AMPK family of serine/threonine kinases. It is expressed in blood mononuclear cells, stem cells and other tissues, and functions in cell cycle progression and pre-mRNA splicing. Human MELK is 651 amino acids (aa) in length and contains one protein kinase domain (aa 11‑263) and a kinase-associated (KA) 1 domain (aa 602‑651). MELK is activated upon phosphorylation of T167 and S171. There are multiple alternative splice variants. Two show the same 16 aa substitution for the N-terminal 87 and 48 aa, respectively; a third shows an alternate start site at M440, and a fourth shows complex splicing over aa 1‑392. Over aa 341‑470, human MELK shares 56% aa identity with mouse MELK.

  • Long Name:
    Maternal Embryonic Leucine Zipper Kinase
  • Entrez Gene IDs:
    9833 (Human)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.1; hMELK; HPK38; KIAA0175pEg3 kinase; maternal embryonic leucine zipper kinase; MELK; PK38; Protein kinase PK38
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