Human Metabolic Luminex® Performance Premixed Kit

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Luminex Metabolic Assay Standard Curves
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Human Metabolic Luminex® Performance Premixed Kit Summary

Panel Luminex Performance Human Metabolic Panel
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

Filter Analytes
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Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.


Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across thirty-eight different serum assays.

  n=40 (20x2 levels) n=25
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
Active GLP-1 5.31 8.72
Active Peptide YY 3.64 7.60
CCL2/JE/MCP-1 3.16 6.11
C-Peptide 8.81 12.1
Glucagon 6.37 10.3
IFN-gamma 3.65 7.19
IL-6 3.61 7.22
Insulin 5.71 7.93
Leptin/OB 4.22 11.0
Pancreatic Polypeptide/PP 9.20 14.7
TNF-alpha 4.13 8.36
Total Ghrelin 6.75 12.4


Linearity - The data represents mean spiked or natural linearity in serum matrix samples.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.

Analyte N Average %
Active GLP-1 8 129
Active Peptide YY 8 100
CCL2/JE/MCP-1 8 126
C-Peptide* 4 125
Glucagon 8 111
IFN-gamma 8 144
IL-6 8 139
Insulin* 4 127
Leptin/OB* 4 117
Pacreatic Polypeptide/PP 8 168
TNF-alpha 8 151
Total Ghrelin 8 172
*Natural linearity
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

Multiplex Immunoassay Luminex Metabolic Assay Standard Curves View Larger

Luminex Metabolic Assay Standard Curves

Multiplex Immunoassay Luminex Metabolic Assay P800 Sample Values View Larger

Luminex Metabolic Assay Serum Sample Values P800 EDTA samples from 20 apparently healthy individuals were run on the Human Metabolic Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Background: Metabolic Assay Kits

Metabolic syndrome describes a collection of conditions associated with elevated blood pressure, obesity, and insulin resistance. The International Diabetes Federation (IDF) estimates that 20-25% of the world's population has metabolic syndrome. This diagnosis is associated with a 2-fold increase in the likelihood of a fatal cardiovascular events. The IDF defines metabolic syndrome as central obesity and any two of the following conditions, hypertension (BP>135/85 mmHg), dyslipidemia, and glucose dysregulation. Metabolic biomarker research is required to understand its mechanisms so that this public health crisis can be averted. It is apparent that Metabolic Syndrome involves the complex interplay of a variety of metabolic biomarkers such as hormones and cytokines. Multiplex quantification of metabolic biomarkers may be more revealing than analyzing a single protein. The Human Metabolic Luminex Performance Assay is an excellent tool for simultaneously assessing the levels of multiple human metabolic biomarkers in a single sample.

Alternate Names
Metabolic Assay Kits

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.


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