Detection of Human Methionine Aminopeptidase 2 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Methionine Aminopeptidase 2/METAP2
The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.
Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.
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