Human Methionine Aminopeptidase 2/METAP2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Methionine Aminopeptidase 2/METAP2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human METAP1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase 2/METAP2
    Ala2-Tyr478
    Accession # P50579
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Methionine Aminopeptidase 2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

  • References:
    1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
    2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
    3. Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.
  • Entrez Gene IDs:
    10988 (Human); 56307 (Mouse); 64370 (Rat)
  • Alternate Names:
    Amp2; eIF-2-associated p67 homolog; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; METAP2; Methionine Aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67EC 3.4.11.18; p67eIF2; Peptidase M 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Pig epiblast stem cells depend on activin/nodal signaling for pluripotency and self-renewal.
    Authors: Alberio R, Croxall N, Allegrucci C
    Stem Cells Dev., 2010;19(10):1627-36.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
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