Human/Mouse ASAM Antibody

Catalog # Availability Size / Price Qty
AF7356
AF7356-SP
Detection of Mouse ASAM by Western Blot.
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Human/Mouse ASAM Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse recombinant  ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ASAM
Thr18-Ala234
Accession # Q8R373
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse ASAM by Western Blot. View Larger

Detection of Mouse ASAM by Western Blot.

Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of ASAM in T98G Human Cell Line by Flow Cytometry. View Larger

Detection of ASAM in T98G Human Cell Line by Flow Cytometry.

T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Flow Cytometry Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAM

ASAM (Adipocyte-specific adhesion molecule; also ACAM, CLMP, Asp5 and CXADR-like protein) is a 44-48 kDa member of the CTX family of molecules. It is expressed on adipocytes as well as select epithelial cells such as respiratory and intestinal epithelium, keratinocytes and Sertoli cells. ASAM appears to contribute to tight junction formation, and may promote the transition of preadipocytes to adipocytes. Mature mouse ASAM is a 356 amino acid (aa) type I transmembrane glycoprotein. It contains a 217 aa extracellular region (aa 18-234) that is characterized by the presence of two C2 type Ig-like domains (aa 18-126 and 134-223), and possesses a C-terminal 118 aa cytoplasmic domain. The extracellular region of mouse ASAM (aa 18-234) shares 97% and 100% aa sequence identity with human and rat ASAM, respectively.

Long Name
Adipocyte-specific Adhesion Molecule
Entrez Gene IDs
79827 (Human); 71566 (Mouse); 286939 (Rat)
Alternate Names
ACAM; ACAMCoxsackie- and adenovirus receptor-like membrane protein; Adipocyte adhesion molecule; adipocyte-specific adhesion molecule; ASAM; ASAMCAR-like membrane protein; Asp5; CLMP; CXADR-like membrane protein; FLJ22415

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