Human/Mouse Bcl-6 Antibody

Name: Human/Mouse Bcl-6 Antibody
Brand: R&D Systems
SKU: MAB5046

Catalog #: MAB5046 Datasheet / COA / SDS

Discontinued Product

MAB5046 has been discontinued.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bcl-6 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Bcl-6 in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant mouse (rm) Bcl-6b is observed. No cross-reactivity with rmBcl-2a, rmBcl-x, recombinant human (rh) Bcl-2, -2-L12, -3, -9, 9-2, -10, -11a, -11b, -w, or -xL is observed.

Source

Monoclonal Rat IgG_2B Clone # 603406

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant mouse Bcl-6
Glu522-696
Accession # Q8CB25

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Bcl‑6 in NIH‑3T3 Mouse Cell Line. Bcl-6 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Bcl-6 Monoclonal Antibody (Catalog # MAB5046) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of Bcl-6 antibody in Raji Human Cell Line antibody by Flow Cytometry.

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Detection of Bcl‑6 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Bcl-6 Monoclonal Antibody (Catalog # MAB5046, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-6

Bcl-6 (Bcell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32- 99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518-681). Centrally located are three PEST sequences (aa 336-430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.

Long Name

B Cell Lymphoma/Leukemia 6

Entrez Gene IDs

604 (Human); 12053 (Mouse); 303836 (Rat)

Alternate Names

B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein transcript; Bcl-5; BCL5B-cell lymphoma 5 protein; Bcl6; Bcl-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; LAZ3ZBTB27B-cell lymphoma 6 protein; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27 ; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51ZNF51; zinc finger transcription factor BCL6S; ZNF51