Human/Mouse cIAP-2/HIAP-1 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB817G Datasheet / COA / SDS

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FAB817G-100UG

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Human/Mouse cIAP-2/HIAP-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse cIAP‑2/HIAP‑1 in Western blots. Does not cross-react with HEK293 cells transfected with cIAP-1.

Source

Monoclonal Mouse IgG1 Clone # 315304

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human cIAPâ€‘2/HIAPâ€‘1
Asn2-Ser604
Accession # Q13489

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: cIAP-2/HIAP-1

cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Human cIAP-2 is a 604 amino acid protein that contains 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.

Entrez Gene IDs

330 (Human)

Alternate Names

AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; cIAP2; c-IAP2; cIAP-2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49