Human/Mouse cIAP-2/HIAP-1 Antibody

Catalog # Availability Size / Price Qty
MAB817
MAB817-SP
Detection of Human and Mouse cIAP‑2/HIAP‑1 by Western Blot.
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Product Details
Citations (5)
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Human/Mouse cIAP-2/HIAP-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse cIAP‑2/HIAP‑1 in Western blots. Does not cross-react with HEK293 cells transfected with cIAP-1.
Source
Monoclonal Mouse IgG1 Clone # 315304
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human cIAP‑2/HIAP‑1
Asn2-Ser604
Accession # Q13489
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse cIAP-2/HIAP-1 antibody by Western Blot. View Larger

Detection of Human and Mouse cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse cIAP-2/HIAP-1 Monoclonal Antibody (Catalog # MAB817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Immunohistochemistry cIAP-2/HIAP-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

cIAP‑2/HIAP‑1 in Human Colon Cancer Tissue. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Mouse cIAP-2/ HIAP-1 Monoclonal Antibody (Catalog # MAB817) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.

Immunohistochemistry cIAP-2/HIAP-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

cIAP‑2/HIAP‑1 in Human Colon. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Mouse Anti-Human/Mouse cIAP-2/HIAP-1 Monoclonal Antibody (Catalog # MAB817) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.

Simple Western Detection of Human cIAP-2/HIAP-1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse cIAP‑2/HIAP‑1 Monoclonal Antibody (Catalog # MAB817). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cIAP-2/HIAP-1

cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Human cIAP-2 is a 604 amino acid protein that contains 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.

References
  1. Roy, N. et al. (1997) EMBO J. 23:6914.
  2. Deveraux, Q. et al. (1997) Nature 388:300.
  3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
  4. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
  5. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Entrez Gene IDs
330 (Human)
Alternate Names
AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; cIAP2; c-IAP2; cIAP-2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49

Product Datasheets

Citations for Human/Mouse cIAP-2/HIAP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Close Relationship between cIAP2 and Human ARDS Induced by Severe H7N9 Infection
    Authors: C Qin, XY Sai, XF Qian, Y Wu, LF Zou, HM Wang, T Bian, Z Yan
    Biomed Res Int, 2019;2019(0):2121357.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Dendritic Cell RIPK1 Maintains Immune Homeostasis by Preventing Inflammation and Autoimmunity
    Authors: JA O'Donnell, J Lehman, JE Roderick, D Martinez-M, M Zelic, C Doran, N Hermance, S Lyle, M Pasparakis, KA Fitzgerald, A Marshak-Ro, MA Kelliher
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. BIRC3 is a biomarker of mesenchymal habitat of glioblastoma, and a mediator of survival adaptation in hypoxia-driven glioblastoma habitats
    Authors: D Wang, AE Berglund, RS Kenchappa, RJ MacAulay, JJ Mulé, AB Etame
    Sci Rep, 2017;7(1):9350.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors.
    Authors: Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S
    Int. J. Oncol., 2007;30(5):1163-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Effect of hyperthermia on TRAIL-induced apoptotic death in human colon cancer cells: development of a novel strategy for regional therapy.
    Authors: Yoo J, Lee YJ
    J. Cell. Biochem., 2007;101(3):619-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse cIAP-2/HIAP-1 Antibody

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Human/Mouse cIAP-2/HIAP-1 Antibody
By Anonymous on 09/23/2021
Application: Western Blot Sample Tested: Colon tissue,Tissue Lysates Species: Mouse