Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

R&D Systems | Catalog # DYC835-2

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

219-14000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human, Mouse

Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
View all Caspase-3 ELISA Kits »

Product Summary for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure cleaved Caspase-3 (Asp175) in cell lysates. An immobilized capture antibody specific for Caspase-3 binds both phosphorylated and unphosphorylated Caspase-3. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

Standard Curve

Kit Contents for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Background: Caspase-3

Caspase-3 is a widely expressed dimeric peptidase that is the major executioner caspase, the primary downstream mediator of apoptotic-associated proteolysis. Caspase-3 cleaves multiple substrates including PARP, proIL-16, PKC-gamma and -delta, proCaspases-6, -7, and -9, and beta-Catenin. Normally, it is an inactive, exclusively cytosolic homodimer. During apoptosis, however, proCaspase-3 is activated by cleavage into p20 and p12 subunits, and the p20 subunit is trimmed to yield a p17 subunit. Active Caspase-3 contains two p17 and two p12 subunits.

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

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