Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

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Product Details
Citations (18)
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Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
219.0 - 14,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure cleaved Caspase-3 (Asp175) in cell lysates. An immobilized capture antibody specific for Caspase-3 binds both phosphorylated and unphosphorylated Caspase-3. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Caspase-3

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Entrez Gene IDs:
836 (Human); 12367 (Mouse)
Alternate Names:
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA

Citations for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. 2-Chlorodeoxyadenosine (Cladribine) preferentially inhibits the biological activity of microglial cells
    Authors: F Aybar, M Julia Pere, M Silvina Ma, M Eugenia Sa, M Marrodan, J María Pasq, J Correale
    International immunopharmacology, 2022;105(0):108571.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Ghrelin May Inhibit Inflammatory Response and Apoptosis During Ischemia-Reperfusion Injury
    Authors: N Fukunaga, RVP Ribeiro, V Bissoondat, F Billia, V Rao
    Transplantation Proceedings, 2022;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Sinularin, an Anti-Cancer Agent Causing Mitochondria-Modulated Apoptosis and Cytoskeleton Disruption in Human Hepatocellular Carcinoma
    Authors: CY Ko, PC Shih, PW Huang, YH Lee, YF Chen, MH Tai, CH Liu, ZH Wen, HM Kuo
    International Journal of Molecular Sciences, 2021;22(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice
    Authors: K Liu, J Liu, B Zou, C Li, HJ Zeh, R Kang, G Kroemer, J Huang, D Tang
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study
    Authors: Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang
    Micromachines, 2021;12(10):.
    Species: Human
    Sample Types: Cell Lysates
  6. Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of Flavouring
    Authors: A Nemmar, S Al-Salam, S Beegam, P Yuvaraju, NE Zaaba, J Yasin, BH Ali
    Int J Mol Sci, 2020;21(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Role of Bee Venom and Melittin on Restraining Angiogenesis and Metastasis in gamma-Irradiated Solid Ehrlich Carcinoma-Bearing Mice
    Authors: NM El Bakary, AZ Alsharkawy, ZA Shouaib, EMS Barakat
    Integr Cancer Ther, 2020;19(0):1534735420944.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis
    Authors: AH Shen, MR Borrelli, S Adem, NMD Deleon, RA Patel, S Mascharak, SJ Yen, BY Sun, WL Taylor, M Januszyk, DH Nguyen, A Momeni, GC Gurtner, MT Longaker, DC Wan
    Sci Rep, 2020;10(1):12346.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Chronic Systemic Inflammation Exacerbates Neurotoxicity in a Parkinson's Disease Model
    Authors: P Ugalde-Muñ, I Fetter-Pru, L Navarro, E García, A Chavarría
    Oxid Med Cell Longev, 2020;2020(0):4807179.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Modulation of PARP activity by Monomethylarsonous (MMA+3) acid and uranium in mouse thymus
    Authors: S Medina, X Zhou, FT Lauer, H Zhang, KJ Liu, J Lewis, SW Burchiel
    Toxicology and Applied Pharmacology, 2020;411(0):115362.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
    Authors: A Nemmar, S Al-Salam, S Beegam, P Yuvaraju, BH Ali
    Oxid Med Cell Longev, 2020;2020(0):6450450.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Gum Arabic Ameliorates Impaired Coagulation and Cardiotoxicity Induced by Water-Pipe Smoke Exposure in Mice
    Authors: A Nemmar, S Al-Salam, S Beegam, P Yuvaraju, BH Ali
    Front Physiol, 2019;10(0):53.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3
    Authors: S Al-Salam, S Hashmi
    Cell. Physiol. Biochem., 2018;50(3):1123-1139.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Mangiferin from Pueraria tuberosa reduces inflammation via inactivation of NLRP3 inflammasome
    Authors: RK Bulugonda, KA Kumar, D Gangappa, H Beeda, GH Philip, D Muralidhar, SM Faisal
    Sci Rep, 2017;7(0):42683.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Hepatoprotective effect of sitagliptin against methotrexate induced liver toxicity
    Authors: HM Abo-Haded, MA Elkablawy, Z Al-Johani, O Al-Ahmadi, DS El-Agamy
    PLoS ONE, 2017;12(3):e0174295.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. miR-30e* is overexpressed in prostate cancer and promotes NF-?B-mediated proliferation and tumor growth
    Authors: SM Egan, E Karasik, L Ellis, SO Gollnick
    Oncotarget, 2017;8(40):67626-67638.
    Species: Mouse
    Sample Types: Cell Lysates
  17. Preconditioning of microglia by alpha-synuclein strongly affects the response induced by toll-like receptor (TLR) stimulation.
    Authors: Roodveldt C, Labrador-Garrido A, Gonzalez-Rey E, Lachaud C, Guilliams T, Fernandez-Montesinos R, Benitez-Rondan A, Robledo G, Hmadcha A, Delgado M, Dobson C, Pozo D
    PLoS ONE, 2013;8(11):e79160.
    Species: Mouse
    Sample Types: Cell Lysates
  18. Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
    Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
    Front Immunol, 0;13(0):933329.
    Species: Complex Species Category
    Sample Types: Cell Lysates


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