DLL4 Antibody (207822)

R&D Systems | Catalog # MAB1389

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 207822
View all DLL4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Ser28-Pro525
Accession # Q9JI71

Specificity

Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant human DLL4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for DLL4 Antibody (207822)

DLL4 antibody in Mouse Embryonic Heart by Immunohistochemistry (IHC-Fr).

DLL4 in Mouse Embryonic Heart.

DLL4 was detected in immersion fixed frozen sections of mouse embryonic heart using Rat Anti-Human/Mouse DLL4 Monoclonal Antibody (Catalog # MAB1389) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of DLL4 by Western Blot

Detection of DLL4 by Western Blot

mDia1is essential for CCN1-induced Cdc42 activation in tip cell formation.(A) HUVECs were treated with PBS (Control) or CCN1 (10 ng/mL) for 24 hr, and total RNA was used for the detection of DIAPH1 (mDia1) mRNA expression by qRT-PCR. *p<0.001 vs. Control. (B) After transfection with DIAPH1 WT full length and DIAPH1 double-negative mutant plasmids, HUVECs were treated with CCN1 and immunostained with anti-vinculin antibody (red) and phalloidin (green) to visualise filopodia. Scale bar = 10 µm. (C, D) HUVECs transfected with DIAPH1 WT full length and DIAPH1 double-negative mutant plasmids were treated with CCN1 (10 ng/mL) for 30 min, and active Cdc42 was assessed by western blotting analysis (C) and visualised by p-N-Wasp (D). Fold changes were noted under each protein band. (E) After HUVECs were transfected with DIAPH1 WT full length and DIAPH1 DN3 mutant plasmid, starved cells for 16 hr were treated with CCN1 10 ng/ml for 1 hr and detected YAP/TAZ by IF. Scale bar = 100 µm.10.7554/eLife.46012.012Figure 4—source data 1.Source data for Figure 4C.Source data for Figure 4C. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31429823), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of DLL4 by Western Blot

Detection of DLL4 by Western Blot

CCN1 stimulates tip cell activity in ECs. (G) Western blotting analysis of VEGFR2, DLL4, and SOX17 protein levels in the same samples as used in F. beta -actin was used as an internal control. Fold increase was indicated as a number below each band. (H) Luciferase assays in HUVECs transfected with DLL4-Luc reporter vector and treated with CCN1 or VEGF (10 ng/mL) for 24 hr. Luciferase activity was normalised to Renilla luminescence. *p<0.001 vs. Control.10.7554/eLife.46012.005Figure 2—source data 1.Source data for Figure 2G.Source data for Figure 2G. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31429823), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for DLL4 Antibody (207822)

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic heart

Western Blot

1 µg/mL
Sample: Recombinant Mouse DLL4 (Catalog # 1389-D4)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB1389 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References

  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, T. et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Long Name

Delta-like 4

Alternate Names

delta 4, Delta 4 precursor, delta ligand 4, delta4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, Drosophila Delta homolog 4, hdelta2, MGC126344, notch ligand delta-2, notch ligand DLL4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Gene Symbol

DLL4

UniProt

Q9JI71

Additional DLL4 Products

Product Documents for DLL4 Antibody (207822)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for DLL4 Antibody (207822)

For research use only

Related Research Areas

Cancer Stem Cell Markers
Notch Pathway

Citations for DLL4 Antibody (207822)

Customer Reviews for DLL4 Antibody (207822) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse DLL4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 04/08/2022
    DLL4 Antibody (207822) MAB1389
  • Human/Mouse DLL4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 09/03/2021
    DLL4 Antibody (207822) MAB1389
  • Human/Mouse DLL4 Antibody
    Name: Simon Liu
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Bladder cancer tissue
    Species: Human
    Verified Customer | Posted 12/10/2017
    DLL4 Antibody (207822) MAB1389

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail