Human/Mouse Doc2 alpha Antibody

Catalog # Availability Size / Price Qty
AF7904
AF7904-SP
Detection of Human and Mouse Doc2 alpha  by Western Blot.
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Human/Mouse Doc2 alpha Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Doc2 alpha in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Doc2 beta is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Doc2 alpha
Met1-Lys114 (Gly48Ser)
Accession # Q14183
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Doc2 alpha  by Western Blot. View Larger

Detection of Human and Mouse Doc2 alpha by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, P19 mouse embryonal carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Doc2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doc2 alpha at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Doc2 alpha

DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells, and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13-1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195), and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene, and shares no meaningful aa sequence identity (< 30%) with human Doc2 alpha.

Long Name
Double C2-like Domains, alpha
Entrez Gene IDs
8448 (Human); 13446 (Mouse); 65031 (Rat)
Alternate Names
Doc2 alpha; Doc2; DOC2A; doc2-alpha; double C2-like domain-containing protein alpha; double C2-like domains, alpha

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