Human/Mouse Follistatin Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # AF669AFP647

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Neutralization

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Polyclonal Goat IgG
View all Follistatin Primary Antibodies »

Product Specifications

Specificity

Detects human and mouse Follistatin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse Follistatin Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. The recombinant human FS-300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS-288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. The binding affinity of R&D Systems’ FS-300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice. Over aa 30-329, human Follistatin shares 97% aa identity with mouse Follistatin.

References

  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835

Alternate Names

FS, FST

Entrez Gene IDs

10468 (Human); 14313 (Mouse)

Gene Symbol

FST

UniProt

P19883

Additional Follistatin Products

Product Documents for Human/Mouse Follistatin Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Follistatin Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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