Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Follistatin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
Gly30-Asp329
Accession # P19883

Specificity

Detects human and mouse Follistatin in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse Follistatin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Follistatin 300 aa 30-329 (Catalog # 669-FO) and Recombinant Mouse Follistatin 288 (FS-288) (Catalog # 769-FS)

Reviewed Applications

Read 1 review rated 5 using BAF669 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high-affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. FS-288 binds with high-affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice.

References

  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835

Alternate Names

FS, FST

Entrez Gene IDs

10468 (Human); 14313 (Mouse)

Gene Symbol

FST

UniProt

P19883

Additional Follistatin Products

Product Documents for Human/Mouse Follistatin Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Follistatin Biotinylated Antibody

For research use only

Citations for Human/Mouse Follistatin Biotinylated Antibody

Customer Reviews for Human/Mouse Follistatin Biotinylated Antibody (1)

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  • Human/Mouse Follistatin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    Human/Mouse Follistatin Biotinylated Antibody BAF669

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