Human/Mouse Follistatin Antibody

Catalog # Availability Size / Price Qty
Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody.
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Product Details
Citations (8)
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Reviews (1)

Human/Mouse Follistatin Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse Follistatin in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human (rh) Follistatin
Accession # P19883
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human (rh) Follistatin 300 aa 30-329 (Catalog # 669-FO)
5-15 µg/mL
See below
Measured by its ability to neutralize Follistatin inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. At 6 μg/mL, this antibody will neutralize >60% of rhFollistatin 300 bioactivity on K562 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody. View Larger

Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody. Recombinant Human Follistatin 300 (Catalog # 669-FO) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin 300 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669). At 6 µg/mL, this antibody will neutralize > 60% of rhFollistatin 300 bioactivity.

Immunohistochemistry Follistatin antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

Follistatin in Human Breast. Follistatin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. The recombinant human FS-300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS-288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. The binding affinity of R&D Systems’ FS-300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice. Over aa 30-329, human Follistatin shares 97% aa identity with mouse Follistatin.

  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

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Citations for Human/Mouse Follistatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Gene therapy for follistatin mitigates systemic metabolic inflammation and post-traumatic arthritis in high-fat diet-induced obesity
    Authors: R Tang, NS Harasymowi, CL Wu, KH Collins, YR Choi, SJ Oswald, F Guilak
    Sci Adv, 2020;6(19):eaaz7492.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone
    Authors: A Iskenderia, N Liu, Q Deng, Y Huang, C Shen, K Palmieri, R Crooker, D Lundberg, N Kastrapeli, B Pescatore, A Romashko, J Dumas, R Comeau, A Norton, J Pan, H Rong, K Derakhchan, DE Ehmann
    Skelet Muscle, 2018;8(1):34.
  3. Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle
    Authors: L Formicola, A Pannérec, RM Correra, B Gayraud-Mo, D Ollitrault, V Besson, S Tajbakhsh, J Lachey, JS Seehra, G Marazzi, DA Sassoon
    Front Physiol, 2018;9(0):515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer
    Authors: DD Seachrist, ST Sizemore, E Johnson, FW Abdul-Kari, KL Weber Bonk, RA Keri
    Breast Cancer Res., 2017;19(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
    Authors: L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly
    Respir. Res., 2017;18(1):152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
    Authors: Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C
    PLoS ONE, 2015;10(6):e0128564.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR.
    Authors: Diel P, Schiffer T, Geisler S, Hertrampf T, Mosler S, Schulz S, Wintgens KF, Adler M
    Mol. Cell. Endocrinol., 2010;330(1):1-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  8. Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
    Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
    J. Bone Miner. Res., 2004;19(8):1302-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization


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Human/Mouse Follistatin Antibody
By Anonymous on 07/08/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human

incubated 1hr at 37C. diluted in reagent diluent 2