Human/Mouse Follistatin Antibody

Catalog # Availability Size / Price Qty
AF669
AF669-SP
Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody.
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Product Details
Citations (11)
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Human/Mouse Follistatin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Follistatin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human (rh) Follistatin
Gly30-Asp329
Accession # P19883
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human (rh) Follistatin 300 aa 30-329 (Catalog # 669-FO)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Follistatin inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. At 6 μg/mL, this antibody will neutralize >60% of rhFollistatin 300 bioactivity on K562 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody. View Larger

Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody. Recombinant Human Follistatin 300 (Catalog # 669-FO) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin 300 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669). At 6 µg/mL, this antibody will neutralize > 60% of rhFollistatin 300 bioactivity.

Immunohistochemistry Follistatin antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

Follistatin in Human Breast. Follistatin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Detection of Mouse Follistatin by Immunohistochemistry View Larger

Detection of Mouse Follistatin by Immunohistochemistry Follistatin (FST) expression is repressed in mouse mammary tumors and is restored using an Fst-expressing transgene. a Endogenous Fst expression in normal mouse mammary glands compared with human epidermal growth factor receptor 2, receptor tyrosine protein kinase erbB-2, proto-oncogene Neu (Her2/Neu)-induced mammary tumors (*p < 0.05). b Immunohistochemical analysis of endogenous FST expression in mouse mammary epithelia and tumors. cFst expression in Neu (single) and Neu/FST (bitransgenic) tumors. Fst messenger RNA expression was assessed by quantitative reverse transcription-polymerase chain reaction (**p < 0.01). d Hematoxylin and eosin (H&E)-stained mammary tumors from Neu (single) and Neu/FST (bitransgenic) mice are shown in the upper two panels. Immunohistochemistry for total (endogenous and transgenic) FST expression in lower two panels in single (left) and bitransgenic (right) tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28583174), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse Follistatin by Immunohistochemistry View Larger

Detection of Mouse Follistatin by Immunohistochemistry Follistatin (FST) expression is repressed in mouse mammary tumors and is restored using an Fst-expressing transgene. a Endogenous Fst expression in normal mouse mammary glands compared with human epidermal growth factor receptor 2, receptor tyrosine protein kinase erbB-2, proto-oncogene Neu (Her2/Neu)-induced mammary tumors (*p < 0.05). b Immunohistochemical analysis of endogenous FST expression in mouse mammary epithelia and tumors. cFst expression in Neu (single) and Neu/FST (bitransgenic) tumors. Fst messenger RNA expression was assessed by quantitative reverse transcription-polymerase chain reaction (**p < 0.01). d Hematoxylin and eosin (H&E)-stained mammary tumors from Neu (single) and Neu/FST (bitransgenic) mice are shown in the upper two panels. Immunohistochemistry for total (endogenous and transgenic) FST expression in lower two panels in single (left) and bitransgenic (right) tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28583174), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. The recombinant human FS-300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS-288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. The binding affinity of R&D Systems’ FS-300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice. Over aa 30-329, human Follistatin shares 97% aa identity with mouse Follistatin.

References
  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

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Citations for Human/Mouse Follistatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
    Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
    bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
    Authors: Salemi S, Schori L, Gerwinn T et al.
    International Journal of Molecular Sciences
    Species: Human
    Sample Types: Protein
    Applications: Simple Western
  3. Gene therapy for follistatin mitigates systemic metabolic inflammation and post-traumatic arthritis in high-fat diet-induced obesity
    Authors: R Tang, NS Harasymowi, CL Wu, KH Collins, YR Choi, SJ Oswald, F Guilak
    Sci Adv, 2020-05-08;6(19):eaaz7492.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone
    Authors: A Iskenderia, N Liu, Q Deng, Y Huang, C Shen, K Palmieri, R Crooker, D Lundberg, N Kastrapeli, B Pescatore, A Romashko, J Dumas, R Comeau, A Norton, J Pan, H Rong, K Derakhchan, DE Ehmann
    Skelet Muscle, 2018-10-27;8(1):34.
  5. Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle
    Authors: L Formicola, A Pannérec, RM Correra, B Gayraud-Mo, D Ollitrault, V Besson, S Tajbakhsh, J Lachey, JS Seehra, G Marazzi, DA Sassoon
    Front Physiol, 2018-05-24;9(0):515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
    Authors: L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly
    Respir. Res., 2017-08-09;18(1):152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer
    Authors: DD Seachrist, ST Sizemore, E Johnson, FW Abdul-Kari, KL Weber Bonk, RA Keri
    Breast Cancer Res., 2017-06-05;19(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
    Authors: Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C
    PLoS ONE, 2015-06-01;10(6):e0128564.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta.
    Authors: Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A
    Eur J Immunol, 2015-04-17;45(6):1783-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR.
    Authors: Diel P, Schiffer T, Geisler S, Hertrampf T, Mosler S, Schulz S, Wintgens KF, Adler M
    Mol. Cell. Endocrinol., 2010-08-27;330(1):1-9.
    Species: Human
    Sample Types: Recombinant Protein, Serum
    Applications: ELISA Development, Immuno-PCR, Western Blot
  11. Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
    Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
    J. Bone Miner. Res., 2004-05-03;19(8):1302-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization

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Human/Mouse Follistatin Antibody
By Anonymous on 07/08/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human

incubated 1hr at 37C. diluted in reagent diluent 2