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Human/Mouse Glucagon Alexa Fluor® 647-conjugated Antibody

IC1249R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1249R-100UG
Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Glucagon Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Glucagon.
Source
Monoclonal Mouse IgG2A Clone # 181402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Glucagon synthetic peptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Glucagon antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor® 647-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Entrez Gene IDs
2641 (Human); 14526 (Mouse); 24952 (Rat)
Alternate Names
GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP

Product Datasheets

Product Datasheet
COA
SDS

FAQs

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