Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry.
beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor® 647-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Glucagon is a 29 amino acid residue pancreatic peptide hormone. It
stimulates gluconeogenesis and glycogenolysis to counter the
glucose-lowering action of insulin.
Have you used Human/Mouse Glucagon Alexa Fluor® 647-conjugated Antibody?
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