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Human/Mouse Glucagon Antibody

Catalog #: MAB1249
16 Citations 2 Reviews Datasheet / COA / SDS
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MAB1249
MAB1249-SP

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Glucagon in beta TC-6 Mouse Cell Line.
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Product Details
Citations (16)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Glucagon Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Glucagon.
Source
Monoclonal Mouse IgG2A Clone # 181402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Glucagon synthetic peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Glucagon antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Glucagon in beta TC-6 Mouse Cell Line. Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Glucagon antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Entrez Gene IDs
2641 (Human); 14526 (Mouse); 24952 (Rat)
Alternate Names
GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP

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Citations for Human/Mouse Glucagon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Pancreatic Alpha-Cells Contribute Together With Beta-Cells to CXCL10 Expression in Type 1 Diabetes
    Authors: Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Giada Licata, Lars Krogvold, Lorella Marselli et al.
    Front Endocrinol (Lausanne)
  2. Characterization of the expression, localization, and secretion of PANDER in alpha-cells.
    Authors: Carnegie JR, Robert-Cooperman CE, Wu J et al.
    Mol Cell Endocrinol
  3. Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors
    Authors: Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Daniela Fignani, Giada Licata, Caterina Formichi et al.
    Frontiers in Immunology
  4. Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans
    Authors: Ng, XW;DiGruccio, MR;Kong, C;Lee, J;Piston, DW;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. An orally available compound suppresses glucagon hypersecretion and normalizes hyperglycemia in type 1 diabetes
    Authors: Asadi, F;Gunawardana, SC;Dolle, RE;Piston, DW;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunofluorescence
  6. Regulation of CTCF loop formation during pancreatic cell differentiation
    Authors: Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Roles of FoxM1-driven basal beta-cell proliferation in maintenance of beta-cell mass and glucose tolerance during adulthood
    Authors: M Kohata, J Imai, T Izumi, J Yamamoto, Y Kawana, A Endo, H Sugawara, J Seiko, H Kubo, H Kawamura, T Sato, S Osaka, Y Munakata, Y Asai, S Kodama, K Takahashi, K Kaneko, H Katagiri
    Journal of diabetes investigation, 2022-07-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
    Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
    Cell Reports, 2022-04-12;39(2):110670.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
    Applications: Neutralization
  9. Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans
    Authors: XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu
    Cell Research, 2021-03-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide
    Authors: P Rattanaamn, YM Roshorm, C Wilasrusme, N Proprom, B Ongphiphad, SC Talchai
    Heliyon, 2020-09-15;6(9):e04951.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Abnormal neutrophil signature in the blood and pancreas of presymptomatic and symptomatic type 1 diabetes
    Authors: F Vecchio, N Lo Buono, A Stabilini, L Nigi, MJ Dufort, S Geyer, PM Rancoita, F Cugnata, A Mandelli, A Valle, P Leete, F Mancarella, PS Linsley, L Krogvold, KC Herold, HE Larsson, SJ Richardson, NG Morgan, K Dahl-Jørge, G Sebastiani, F Dotta, E Bosi, M Battaglia
    JCI Insight, 2018-09-20;3(18):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
    Authors: Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M
    PLoS ONE, 2015-08-19;10(8):e0133862.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013-02-04;46(4):259-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. A local glucagon-like peptide 1 (GLP-1) system in human pancreatic islets
    Authors: P. Marchetti, R. Lupi, M. Bugliani, C. L. Kirkpatrick, G. Sebastiani, F. A. Grieco et al.
    Diabetologia
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  15. Stem cells derived from human fetal membranes display multilineage differentiation potential.
    Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
    Biol. Reprod., 2007-05-09;77(3):577-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. A local glucagon-like peptide 1 (GLP-1) system in human pancreatic islets
    Authors: P. Marchetti, R. Lupi, M. Bugliani, C. L. Kirkpatrick, G. Sebastiani, F. A. Grieco et al.
    Diabetologia

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Reviews for Human/Mouse Glucagon Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse Glucagon Antibody
By Anonymous on 04/17/2018
Application: Flow Sample Tested: monoclonal IgG Species: Human
Flow Cytometry Human/Mouse Glucagon Antibody MAB1249
Human/Mouse Glucagon Antibody
By Anonymous on 12/08/2017
Application: Func Sample Tested: mouse igg Species: Mouse