Human/Mouse Glucagon Antibody

Catalog # Availability Size / Price Qty
Glucagon in beta TC-6 Mouse Cell Line.
2 Images
Product Details
Citations (8)
Supplemental Products
Reviews (2)

Human/Mouse Glucagon Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse Glucagon.
Monoclonal Mouse IgG2A Clone # 181402
Protein A or G purified from hybridoma culture supernatant
Human Glucagon synthetic peptide
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Glucagon antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Glucagon in beta TC-6 Mouse Cell Line. Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Glucagon antibody in  beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Entrez Gene IDs
2641 (Human); 14526 (Mouse); 24952 (Rat)
Alternate Names
GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP

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Citations for Human/Mouse Glucagon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
    Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
    Cell Reports, 2022;39(2):110670.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
    Applications: Neutralization
  2. Roles of FoxM1-driven basal beta-cell proliferation in maintenance of beta-cell mass and glucose tolerance during adulthood
    Authors: M Kohata, J Imai, T Izumi, J Yamamoto, Y Kawana, A Endo, H Sugawara, J Seiko, H Kubo, H Kawamura, T Sato, S Osaka, Y Munakata, Y Asai, S Kodama, K Takahashi, K Kaneko, H Katagiri
    Journal of diabetes investigation, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans
    Authors: XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu
    Cell Research, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide
    Authors: P Rattanaamn, YM Roshorm, C Wilasrusme, N Proprom, B Ongphiphad, SC Talchai
    Heliyon, 2020;6(9):e04951.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Abnormal neutrophil signature in the blood and pancreas of presymptomatic and symptomatic type 1 diabetes
    Authors: F Vecchio, N Lo Buono, A Stabilini, L Nigi, MJ Dufort, S Geyer, PM Rancoita, F Cugnata, A Mandelli, A Valle, P Leete, F Mancarella, PS Linsley, L Krogvold, KC Herold, HE Larsson, SJ Richardson, NG Morgan, K Dahl-Jørge, G Sebastiani, F Dotta, E Bosi,,, M Battaglia
    JCI Insight, 2018;3(18):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
    Authors: Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M
    PLoS ONE, 2015;10(8):e0133862.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013;46(4):259-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Stem cells derived from human fetal membranes display multilineage differentiation potential.
    Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
    Biol. Reprod., 2007;77(3):577-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Glucagon Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse Glucagon Antibody
By Anonymous on 04/17/2018
Application: Flow Sample Tested: monoclonal IgG Species: Human

Human/Mouse Glucagon Antibody
By Anonymous on 12/08/2017
Application: Func Sample Tested: mouse igg Species: Mouse