Glucagon Antibody (181402)

R&D Systems | Catalog # MAB1249

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 181402
View all Glucagon Primary Antibodies »

Product Specifications

Immunogen

Human Glucagon synthetic peptide

Specificity

Detects human and mouse Glucagon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Glucagon Antibody (181402)

Glucagon antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Glucagon in beta TC-6 Mouse Cell Line.

Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Glucagon antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry.

Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry.

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Glucagon Antibody (181402)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: beta TC‑6 mouse beta cell insulinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB1249 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Alternate Names

GCG, GRPP

Entrez Gene IDs

2641 (Human); 14526 (Mouse); 24952 (Rat)

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (181402)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Glucagon Antibody (181402)

For research use only

Citations for Glucagon Antibody (181402)

Customer Reviews for Glucagon Antibody (181402) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse Glucagon Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: monoclonal IgG
    Species: Human
    Verified Customer | Posted 04/17/2018
    Glucagon Antibody (181402) MAB1249
  • Human/Mouse Glucagon Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: mouse igg
    Species: Mouse
    Verified Customer | Posted 12/08/2017
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22069255
    Species: Mouse
    Verified Customer | Posted 02/04/2015

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Protocols

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