Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 181402
View all Glucagon Primary Antibodies »

Product Specifications

Immunogen

Human Glucagon synthetic peptide

Specificity

Detects human and mouse Glucagon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse Glucagon Antibody

Glucagon antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Glucagon in beta TC-6 Mouse Cell Line.

Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry.

Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry.

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Glucagon Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: beta TC‑6 mouse beta cell insulinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB1249 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Alternate Names

GCG, GRPP

Entrez Gene IDs

2641 (Human); 14526 (Mouse); 24952 (Rat)

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Human/Mouse Glucagon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Glucagon Antibody

For research use only

Citations for Human/Mouse Glucagon Antibody

Customer Reviews for Human/Mouse Glucagon Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse Glucagon Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: monoclonal IgG
    Species: Human
    Verified Customer | Posted 04/17/2018
    Human/Mouse Glucagon Antibody MAB1249
  • Human/Mouse Glucagon Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: mouse igg
    Species: Mouse
    Verified Customer | Posted 12/08/2017
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22069255
    Species: Mouse
    Verified Customer | Posted 02/04/2015

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Protocols

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