Human/Mouse Glucagon Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Glucagon.
  • Source
    Monoclonal Mouse IgG2A Clone # 181402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human Glucagon synthetic peptide
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glucagon
Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.
  • Entrez Gene IDs:
    2641 (Human); 14526 (Mouse); 24952 (Rat)
  • Alternate Names:
    GCG; glicentin-related polypeptide; Glucagon; GRPP
Related Research Areas

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