Human/Mouse Golgi Glycoprotein 1/GLG1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 by Western Blot.
2 Images
Product Details
Supplemental Products

Human/Mouse Golgi Glycoprotein 1/GLG1 Antibody Summary

Species Reactivity
Human, Mouse
Detects human Golgi Glycoprotein 1/GLG1 in direct ELISA. Detects human and mouse GLG1 in Western Blots.
Monoclonal Mouse IgG1 Clone # 858238
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Golgi Glycoprotein 1/GLG1
Accession # Q92896
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 antibody by Western Blot. View Larger

Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Golgi Glycoprotein 1/GLG1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Golgi Glycoprotein 1/GLG1 in HeLa Human Cell Line. Golgi Glycoprotein 1/GLG1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Golgi Glycoprotein 1/GLG1

GLG1 (Golgi complex-Localized Glycoprotein 1), also known as CFR1, E-Selectin Ligand-1/ESL-1, MG-160 and Cys-rich FGF Receptor, is a 150-160 kDa (reducing; 130 kDa nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents; not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL-1 collaborates with PSGL-1 to mediate leukocyte binding to endothelial cell surfaces. PSGL-1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra-Golgi receptor for multiple FGFs, including FGF-1, -2, -4, -18 and possibly -3, and as a component of an unusual latent TGF-beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa 30-1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly-Gln segment followed by 16 Cys-rich repeats (aa 116-1101). There are three potential isoform variants, one of which possess a 24 aa extension at the C‑terminus, a second that couples the aforementioned C‑terminal extension to a deletion of aa 147-157, and a third that contains a 14 aa substitution for aa 685-1179. It is suggested that the longer C‑terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa 1048‑1145, human and mouse are identical in aa sequence.

Entrez Gene IDs
2734 (Human); 20340 (Mouse); 29476 (Rat)
Alternate Names
CFR1; CFR-1; cysteine-rich fibroblast growth factor receptor; DKFZp686L08213; E-selectin ligand 1; ESL1; ESL-1; FLJ23319; FLJ23967; FLJ32797; GLG1; Golgi apparatus protein 1; Golgi Glycoprotein 1; golgi sialoglycoprotein MG-160; MG160; MG-160

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Golgi Glycoprotein 1/GLG1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse Golgi Glycoprotein 1/GLG1 Antibody and earn rewards!

Have you used Human/Mouse Golgi Glycoprotein 1/GLG1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review