Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of HIF‑1 alpha  in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Recognizes human and mouse HIF-1 alpha in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 241812
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HIF-1 alpha
Accession # Q16665.1
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

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Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Obesity enhances antiviral immunity in the genital mucosa through a microbiota-mediated effect on gammadelta T�cells
    Authors: JH Park, I Kang, HC Kim, Y Lee, SK Lee, HK Lee
    Cell Reports, 2022;41(6):111594.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Epithelial-mesenchymal transition and metastatic ability of CD133+ colorectal cancer stem-like cells under hypoxia
    Authors: M Okada, K Kawai, H Sonoda, H Shiratori, J Kishikawa, H Nagata, H Nozawa, K Sasaki, M Kaneko, K Murono, S Emoto, Y Iida, H Ishii, Y Yokoyama, H Anzai, K Hasegawa, S Ishihara
    Oncol Lett, 2021;21(1):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD74 is a regulator of hematopoietic stem cell maintenance
    Authors: S Becker-Her, M Rozenberg, C Hillel-Kar, N Gil-Yarom, MP Kramer, A Barak, L Sever, K David, L Radomir, H Lewinsky, M Levi, G Friedlande, R Bucala, A Peled, I Shachar
    PloS Biology, 2021;19(3):e3001121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells
    Authors: Y Sun, X Lin, B Liu, Y Zhang, W Li, S Zhang, F He, H Tian, X Zhu, X Liu, J Wu, J Cai, M Li
    Science Advances, 2021;7(52):eabj6877.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Silibinin down-regulates PD-L1 expression in nasopharyngeal carcinoma by interfering with tumor cell glycolytic metabolism
    Authors: LS Sellam, R Zappasodi, F Chettibi, D Djennaoui, N Yahi-Ait M, ZC Amir-Tidad, C Touil-Bouk, W Ouahioune, JD Wolchok, T Merghoub, M Bourouba
    Arch. Biochem. Biophys., 2020;690(0):108479.
    Species: Human
    Sample Types: Cells
    Applications: Flow Cytometry
  6. Lactate released by inflammatory bone marrow neutrophils induces their mobilization via endothelial GPR81 signaling
    Authors: E Khatib-Mas, S Bhattachar, H Massalha, A Biram, K Golan, O Kollet, A Kumari, F Avemaria, E Petrovich-, S Gur-Cohen, T Itkin, I Brandenbur, A Spiegel, Z Shulman, Z Gerhart-Hi, S Itzkovitz, M Gunzer, S Offermanns, R Alon, A Ariel, T Lapidot
    Nat Commun, 2020;11(1):3547.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Induction of HIF-1? by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation
    Authors: G Duette, P Pereyra Ge, J Rubione, PS Perez, AL Landay, SM Crowe, Z Liao, KW Witwer, MP Holgado, J Salido, J Geffner, O Sued, CS Palmer, M Ostrowski
    MBio, 2018;9(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1?-dependent glycolysis
    Authors: Y Lu, H Liu, Y Bi, H Yang, Y Li, J Wang, Z Zhang, Y Wang, C Li, A Jia, L Han, Y Hu, Y Zhao, R Wang, G Liu
    Cell. Mol. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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