Detection of IL‑12 R beta 2 in Human TH1 cells by Flow Cytometry.
Human CD4+ peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and Mouse Anti-Human/Mouse IL‑12 R beta 2 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1959C). Quadrant markers were set based on control antibody staining using Mouse IgG1 PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 2
Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).
The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).
Trinchieri, G. et al. (2003) Immunity 19:641.
Brombacher, F. et al. (2003) Trends in Immunol. 23:207.
Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
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