Human/Mouse Integrin  alpha 11 Antibody MAB4235: R&D Systems

Human/Mouse Integrin  alpha 11 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human Integrin alpha 2, alpha 10, alpha X, or alpha L.
  • Source
    Monoclonal Rat IgG1 Clone # 396214
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha 11
    Phe23-Pro1142 (Leu972Pro, Val1030 del)
    Accession # EAW77820
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Integrin alpha 11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human heart tissue. PVDF membrane was probed with 2 µg/mL of Human/Mouse Integrin  alpha 11 Monoclonal Antibody (Catalog # MAB4235) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin alpha 11 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 11

Integrin alpha 11 (ITGA11) is a 150 kDa transmembrane glycoprotein that associates with the Integrin beta 1/CD29 chain to form a receptor for collagen I and IX. ITGA11 is most highly expressed in adult cardiac and uterine smooth muscle and developing myocytes. The extracellular region contains seven FG-GAP repeats and one VWF‑C domain. Within the extracellular domain, human and mouse ITGA11 share 90% aa sequence identity.

  • Entrez Gene IDs:
    22801 (Human); 319480 (Mouse); 315744 (Rat)
  • Alternate Names:
    HsT18964; Integrin alpha 11; integrin alpha-11; integrin, alpha 11; ITGA11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency
    Authors: AK Tharkeshwa, J Trekker, W Vermeire, J Pauwels, R Sannerud, DA Priestman, D Te Vruchte, K Vints, P Baatsen, JP Decuypere, H Lu, S Martin, P Vangheluwe, JV Swinnen, L Lagae, F Impens, FM Platt, K Gevaert, W Annaert
    Sci Rep, 2017;7(0):41408.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. PAK proteins and YAP-1 signalling downstream of integrin beta-1 in myofibroblasts promote liver fibrosis
    Nat Commun, 2016;7(0):12502.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  3. Cardiomyocyte contractile status is associated with differences in fibronectin and integrin interactions.
    Authors: Wu X, Sun Z, Foskett A
    Am. J. Physiol. Heart Circ. Physiol., 2010;298(6):H2071-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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