Interferon Regulatory Factor 2 (IRF2) belongs to the interferon regulatory transcription factor family. IRF2 functions as a regulator of type I interferons by influencing cellular proliferation and immune response through transcriptional regulation. IRF2 competitively inhibits IRF1 gene activation, as well as, stimulating transcription on its own. Overexpression of IRF2 in NIH3T3 cells leads to transformation, whereas, loss of IRF2 expression in knock out mice leads to a variety of abnormalities including loss of NK cells due to increased apoptosis.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant mouse IRF2
Val151-Cys349
Accession # P23906
Val151-Cys349
Accession # P23906
Specificity
Detects endogenous human and mouse IRF2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse IRF2 Antibody
Detection of Human/Mouse IRF2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4529) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse IRF2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line
Sample: Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IRF2
Long Name
Interferon Regulatory Factor 2
Alternate Names
DKFZp686F0244, interferon regulatory factor 2, IRF-2
Gene Symbol
IRF2
UniProt
Additional IRF2 Products
Product Documents for Human/Mouse IRF2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse IRF2 Antibody
For research use only
Related Research Areas
Customer Reviews for Human/Mouse IRF2 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse IRF2 Antibody and earn rewards!
Have you used Human/Mouse IRF2 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
