Human/Mouse Kallikrein 7 Antibody Summary
Accession # P49862
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Kallikrein 7 in Human Skin. Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse Kallikrein 7 by Western Blot. Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 7
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268‑PI, 1295-PI, 1669-PI, and 1266-PI).
- Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
- Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
- Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
- Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
Citations for Human/Mouse Kallikrein 7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma
Authors: H Ma, A Hockla, C Mehner, M Coban, N Papo, DC Radisky, ES Radisky
Sci Rep, 2019;9(1):1844.
Sample Types: Cell Lysates
Applications: Western Blot
Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice
Authors: K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita
EMBO Mol Med, 2018;0(0):.
Sample Types: Whole Tissue
Clinical value of protein expression of kallikrein-related peptidase 7 (KLK7) in ovarian cancer.
Authors: Dorn, Julia, Gkazepis, Apostolo, Kotzsch, Matthias, Kremer, Marcus, Propping, Corinna, Mayer, Katharin, Mengele, Karin, Diamandis, Elefther, Kiechle, Marion, Magdolen, Viktor, Schmitt, Manfred
Biol Chem, 2014;395(1):95-107.
Sample Types: Tissue Homogenates
Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
Authors: Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J
Histol Histopathol, 2013;28(1):133-44.
Sample Types: Whole Cells
The extracellular matrix protein fibronectin is a substrate for kallikrein 7.
Authors: Ramani VC, Haun RS
Biochem. Biophys. Res. Commun., 2008;369(4):1169-73.
Sample Types: Recombinant Protein
Applications: Western Blot
Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
Biol. Chem., 2007;388(11):1235-42.
Sample Types: Whole Tissue
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