Detects human and mouse Kallikrein 7 in Western blots. Detects human Kallikrein 7 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 4, rhKallikrein 5, rhKallikrein 8, rhKallikrein 9, rhKallikrein 11, rhKallikrein 13 and rhKallikrein 14 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7 Glu23-His252 Accession # P49862
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human and Mouse Kallikrein 7 by Western Blot.
Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 7 in Human Skin.
Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Kallikrein 7
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268-PI, 1295-PI, 1669-PI, and 1266-PI).
Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
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but also provides information about sample types, species, and experimental conditions.
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