Human/Mouse Kallikrein 7 Antibody AF2624: R&D Systems

Human/Mouse Kallikrein 7 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Kallikrein 7 in Western blots. Detects human Kallikrein 7 in direct ELISAs. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 4, rhKallikrein 5, rhKallikrein 8, rhKallikrein 9, rhKallikrein 11, rhKallikrein 13 and rhKallikrein 14 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
    Glu23-His252
    Accession # P49862
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Kallikrein 7 by Western Blot. Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Kallikrein 7 in Human Skin. Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 7
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268‑PI, 1295-PI, 1669-PI, and 1266-PI).
  • References:
    1. Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
    2. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
    3. Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
    4. Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
    5. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
  • Entrez Gene IDs:
    5650 (Human); 23993 (Mouse)
  • Alternate Names:
    EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); Kallikrein 7; kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Clinical value of protein expression of kallikrein-related peptidase 7 (KLK7) in ovarian cancer.
    Authors: Dorn, Julia, Gkazepis, Apostolo, Kotzsch, Matthias, Kremer, Marcus, Propping, Corinna, Mayer, Katharin, Mengele, Karin, Diamandis, Elefther, Kiechle, Marion, Magdolen, Viktor, Schmitt, Manfred
    Biol Chem, 2014;395(1):95-107.
    Species: Human
    Sample Type: Tissue Lysate
    Application: IHC
  2. Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
    Authors: Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J
    Histol Histopathol, 2013;28(1):133-44.
    Species: Human
    Sample Type: whole cells
    Application: IHC Paraffin-embedded
  3. The extracellular matrix protein fibronectin is a substrate for kallikrein 7.
    Authors: Ramani VC, Haun RS
    Biochem. Biophys. Res. Commun., 2008;369(4):1169-73.
    Species: Human
    Sample Type: Recombinant KLK7
    Application: WB
  4. Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
    Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
    Biol. Chem., 2007;388(11):1235-42.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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