Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kallikrein 7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862

Specificity

Detects human and mouse Kallikrein 7 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Kallikrein 7 Antibody

Detection of Human and Mouse Kallikrein 7 antibody by Western Blot.

Detection of Human and Mouse Kallikrein 7 by Western Blot.

Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kallikrein 7 antibody in Human Skin by Immunohistochemistry (IHC-P).

Kallikrein 7 in Human Skin.

Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Kallikrein 7 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 7 (Catalog # 2624-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human skin tissue and mouse skin tissue

Reviewed Applications

Read 1 review rated 5 using AF2624 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 7

Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268‑PI, 1295-PI, 1669-PI, and 1266-PI).

References

  1. Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
  2. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  3. Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
  4. Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
  5. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.

Alternate Names

KLK7

Entrez Gene IDs

5650 (Human); 23993 (Mouse)

Gene Symbol

KLK7

UniProt

P49862

Additional Kallikrein 7 Products

Product Documents for Human/Mouse Kallikrein 7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Kallikrein 7 Antibody

For research use only

Citations for Human/Mouse Kallikrein 7 Antibody

Customer Reviews for Human/Mouse Kallikrein 7 Antibody (1)

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  • Human/Mouse Kallikrein 7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 03/13/2020
    Human/Mouse Kallikrein 7 Antibody AF2624

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