Human/Mouse Leukotriene A4 Hydrolase/
LTA4H Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Leukotriene A4 Hydrolase/LTA4H in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Leukotriene A4 Hydrolase/LTA4H
    Pro2-Asp611
    Accession # P09960
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Leukotriene A4 Hydrolase/LTA4H by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, CTLL‑2 mouse cytotoxic T cell line, and human tonsil tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leukotriene A4 Hydrolase/LTA4H at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leukotriene A4 Hydrolase/LTA4H

Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, Leukotriene A4 Hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1, 3).

The mature chain of human LTA4H consists of 610 amino acids (residues 2‑611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). R&D Systems rhLTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity.

  • References:
    1. Haeggstrom, J.Z. (2006) J. Biol. Chem. 279:50639.
    2. Orning, L. et al. (1994) J. Biol. Chem. 269:11269.
    3. Chen, X. et al. (2004) Curr. Cancer Drug Targets 4:267.
  • Entrez Gene IDs:
    4048 (Human); 16993 (Mouse); 299732 (Rat)
  • Alternate Names:
    EC 3.3.2.6; Leukotriene A(4) hydrolase; Leukotriene A4 Hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4; LTA4H
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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