LMO4 Antibody

R&D Systems | Catalog # AF3889

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human LMO4
Met1-Cys165
Accession # P61968

Specificity

Detects human and mouse LMO4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for LMO4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human LMO4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LMO4

LMO4 is a 18‑20 kDa member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans‑activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cysteine-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical.

Long Name

LIM Domain Only 4

Alternate Names

Crp3, Etohi4

Entrez Gene IDs

8543 (Human); 16911 (Mouse); 362051 (Rat)

Gene Symbol

LMO4

UniProt

Additional LMO4 Products

Product Documents for LMO4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for LMO4 Antibody

For research use only

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