LMO4 is a 18‑20 kDa member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans‑activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cysteine-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human LMO4
Met1-Cys165
Accession # P61968
Met1-Cys165
Accession # P61968
Specificity
Detects human and mouse LMO4 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for LMO4 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human LMO4
Sample: Recombinant Human LMO4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LMO4
Long Name
LIM Domain Only 4
Alternate Names
Crp3, Etohi4
Gene Symbol
LMO4
UniProt
Additional LMO4 Products
Product Documents for LMO4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for LMO4 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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