Human/Mouse MCPIP1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 604421
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human MCPIP1
    Asp426-Glu599
    Accession # Q5D1E8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse MCPIP1 by Western Blot. Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCPIP1
Human MCP-induced protein (MCPIP), also known as ZC3H12A, is an approximately 66 kDa intracellular protein that contains one RNAse domain (aa 134-290), one zinc finger (aa 301-324), and a proline-rich region (aa 458-536). Within aa 426-599, human MCPIP shares approximately 79% aa sequence identity with mouse and rat MCPIP. Its expression is induced by inflammatory stimulation and cellular stress. It acts to dampen inflammatory responses by promoting the degradation of proinflammatory cytokine mRNAs, inhibiting NFkB activation, and antagonizing TLR signaling. MCPIP exhibits deubiquitinase activity and inhibits the biogenesis of miRNA. It also enhances inflammation-induced angiogenesis, osteocyte and adipocyte differentiation, and the cell death of cardiac myocytes.
  • Long Name:
    MCP-1-induced Protein 1
  • Entrez Gene IDs:
    80149 (Human); 230738 (Mouse); 313587 (Rat)
  • Alternate Names:
    MCPIP1; ZC3H12A; zinc finger CCCH-type containing 12A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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