Human/Mouse MCPIP1 Antibody

Catalog # Availability Size / Price Qty
MAB7875
MAB7875-SP
Detection of Human and Mouse MCPIP1 by Western Blot.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse MCPIP1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 604421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MCPIP1
Asp426-Glu599
Accession # Q5D1E8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse MCPIP1 antibody by Western Blot. View Larger

Detection of Human and Mouse MCPIP1 by Western Blot. Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCPIP1

Human MCP-induced protein (MCPIP), also known as ZC3H12A, is an approximately 66 kDa intracellular protein that contains one RNAse domain (aa 134-290), one zinc finger (aa 301-324), and a proline-rich region (aa 458-536). Within aa 426-599, human MCPIP shares approximately 79% aa sequence identity with mouse and rat MCPIP. Its expression is induced by inflammatory stimulation and cellular stress. It acts to dampen inflammatory responses by promoting the degradation of proinflammatory cytokine mRNAs, inhibiting NFkB activation, and antagonizing TLR signaling. MCPIP exhibits deubiquitinase activity and inhibits the biogenesis of miRNA. It also enhances inflammation-induced angiogenesis, osteocyte and adipocyte differentiation, and the cell death of cardiac myocytes.

Long Name
MCP-1-induced Protein 1
Entrez Gene IDs
80149 (Human); 230738 (Mouse); 313587 (Rat)
Alternate Names
MCPIP1; Reg1; Regnase-1; ZC3H12A; zinc finger CCCH-type containing 12A

Product Datasheets

Citations for Human/Mouse MCPIP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Disrupting Roquin-1 interaction with Regnase-1 induces autoimmunity and enhances antitumor responses
    Authors: G Behrens, SL Edelmann, T Raj, N Kronbeck, T Monecke, E Davydova, EH Wong, L Kifinger, F Giesert, ME Kirmaier, C Hohn, LS de Jonge, MG Pisfil, M Fu, S Theurich, S Feske, N Kawakami, W Wurst, D Niessing, V Heissmeyer
    Nature Immunology, 2021;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. RTEC-intrinsic IL-17-driven inflammatory circuit amplifies antibody-induced glomerulonephritis and is constrained by Regnase-1
    Authors: DD Li, R Bechara, K Ramani, CV Jawale, Y Li, JK Kolls, SL Gaffen, PS Biswas
    JCI Insight, 2021;6(13):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells
    Authors: M Rosenbaum, A Gewies, K Pechloff, C Heuser, T Engleitner, T Gehring, L Hartjes, S Krebs, D Krappmann, M Kriegsmann, W Weichert, R Rad, C Kurts, J Ruland
    Nat Commun, 2019;10(1):2352.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. MALT1 Phosphorylation Controls Activation of T Lymphocytes and Survival of ABC-DLBCL Tumor Cells
    Authors: T Gehring, T Erdmann, M Rahm, C Gra beta, A Flatley, TJ O'Neill, S Woods, I Meininger, O Karayel, K Kutzner, M Grau, H Shinohara, K Lammens, R Feederle, SM Hauck, G Lenz, D Krappmann
    Cell Rep, 2019;29(4):873-888.e10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Malt1 self-cleavage is critical for regulatory T cell homeostasis and anti-tumor immunity in mice
    Authors: M Baens, R Stirparo, Y Lampi, D Verbeke, R Vandepoel, J Cools, P Marynen, CE de Bock, S Bornschein
    Eur. J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Autocleavage of the paracaspase MALT1 at Arg-781 attenuates NF-?B signaling and regulates the growth of activated B-cell like diffuse large B-cell lymphoma cells
    Authors: CH Wu, YH Yang, MR Chen, CH Tsai, AL Cheng, SL Doong
    PLoS ONE, 2018;13(6):e0199779.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation
    Authors: K Seno, Y Munakata, M Sano, R Kawahara-M, H Takahashi, A Ohkuchi, H Iwata, T Kuwayama, K Shirasuna
    Int J Mol Sci, 2018;19(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse MCPIP1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse MCPIP1 Antibody
By Anonymous on 11/11/2021
Application: WB Sample Tested: B lymphocytes Species: Mouse

Human/Mouse MCPIP1 Antibody
By Anonymous on 07/12/2018
Application: WB Sample Tested: T cells Species: Human

Primary T cells were activated with PMA/ionomycin for the time points as shown. Cell lysates were harvested in a NP40-based lysis buffer. 8% SDS-PAGE, transferred to a nitrocellulose membrane by semi-dry transfer machine. Blocked by 5% milk for 30 mins. Antibody dilution was used at 1:500. 680nm Donkey anti-mouse was used as secondary antibody (1:30,000). Image captured by Licor Odyssey. The arrow shows the full length Regnase-1, and the rectangle shows the cleaved product upon TCR stimulation.