Human/Mouse MMP-24/MT5-MMP Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse MMP‑24/MT5-MMP in direct ELISAs and detects human and mouse MMP‑24/MT5-MMP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-9, and the catalytic domains of recombinant human MMP‑14, 15, and 16 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 143908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse MMP‑24/MT5-MMP
    Arg125-Gln296
    Accession # Q9R0S2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human/Mouse MMP‑24/MT5-MMP
  • Flow Cytometry
    0.25 µg/106 cells
    A172 human glioblastoma cell line and bEnd.3 mouse endothelioma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-24/MT5-MMP
MMP-24, also known as MT5-MMP, is a 62-65 kDa member of the M10 metallopeptidase family, a subfamily of MMPs. It is a type I transmembrane glycoprotein that is converted into a 58 kDa active form (amino acids [aa] 129-618) following removal of its propeptide. Proteolytic cleavage after Arg554 generates a 52 kDa active, soluble form that undergoes further cleavage in to 27-34 kDa fragment. MMP-24 has restricted expression, being found on neurons, mast cells, neural stem cells and ependymal cells lining the ventricles of the brain. It is known to cleave chondroitin sulfate, dermatan sulfate, pro-MMP-2, and N-Cadherin. Its activation of MMP-2 likely contributes to neurite outgrowth, while its cleavage of N-Cadherin in-trans between neural stem cells and ependymal initiates stem cell expansion and proliferation. Over amino acids 125-296, mouse MMP-24 shares 100% and 99% aa sequence identity with rat and human MMP-24, respectively.
  • Long Name:
    Matrix Metalloproteinase 24/Membrane Type 5 MMP
  • Entrez Gene IDs:
    10893 (Human); 17391 (Mouse)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 24 (membrane-inserted); matrix metalloproteinase 24 (membrane-inserted); matrix metalloproteinase-24; membrane-type 5 matrix metalloproteinase; Membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP24; MMP-24; MT5MMP; MT5-MMP; MT5-MMPMMP25; MT-MMP 5; MTMMP5; MT-MMP5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 88  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse MMP-24/MT5-MMP Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information