Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MYB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MYB
Lys503-Met640
Accession # P10242

Specificity

Detects human and mouse MYB in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse MYB Antibody

Detection of Human and Mouse MYB antibody by Western Blot.

Detection of Human and Mouse MYB by Western Blot.

Western blot shows lysates of CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse MYB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MYB at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse MYB Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH‑1 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYB

MYB (myeloblastosis protooncogene) is an 80 kDa transcriptional activator. It is found in hematopoietic and intestinal crypt epithelial cells. MYB interacts with p300 and activates multiple genes, including Bcl2, GATA3, and MYC. Although MYB is thought of as a pro-proliferation and antidifferentiation factor, it would now appear that MYB contributes to both T- and B-cell maturation and to cell survival in general. Human MYB is 640 amino acids (aa) in length. It contains three DNA binding, HTH‑myb type domains (aa 35‑193), one transcriptional activation segment (aa 275‑327), and a Leu‑zipper motif (aa 376‑397). There are multiple potential splice forms. One shows an alternate start site at Met189 coupled with a 99 aa substitution for aa 567‑640. Another shows a 121 aa insertion after Ser401. Four show C‑terminal substitutions: a 34 aa substitution for aa 314‑640, a Met substitution for aa 402‑640, a seven aa substitution for aa 317‑640, and a 10 aa substitution for aa 603‑640. Over aa 503‑640, human MYB shares 84% aa identity with mouse MYB.

Long Name

v-myb Myeloblastosis Viral Oncogene Homolog

Alternate Names

Cmyb;c-myb;c-myb_CDS;efg;Transcriptional activator Myb

Entrez Gene IDs

4602 (Human); 17863 (Mouse)

Gene Symbol

MYB

UniProt

P10242 (Human)

Additional MYB Products

Product Documents for Human/Mouse MYB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MYB Antibody

For research use only

Citations for Human/Mouse MYB Antibody

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