NeuroD1 in beta TC-6 Mouse Cell Line.
Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a
basic helix-loop-helix transcription factor. It is expressed during
development in various neurons of the central and peripheral nervous
systems. NeuroD1 is also expressed in beta-cells in the pancreas where
it functions both as a transcriptional activator and repressor.
Neurogenic Differentiation Factor 1
Entrez Gene IDs:
basic helix-loop-helix transcription factor; BETA2; BHF-1; BHLHA3; bHLHa3beta-cell E-box transactivator 2; Class A basic helix-loop-helix protein 3; MODY6; NeuroD; NeuroD1; neurogenic differentiation 1; neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; NIDDM
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