Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NeuroD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562

Specificity

Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NeuroD1 Antibody

NeuroD1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

NeuroD1 in beta TC-6 Mouse Cell Line.

Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse NeuroD1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human NeuroD1

Reviewed Applications

Read 1 review rated 5 using AF2746 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name

Neurogenic Differentiation Factor 1

Alternate Names

BETA2, BHF-1, NIDDM

Entrez Gene IDs

4760 (Human)

Gene Symbol

NEUROD1

UniProt

Q13562

Additional NeuroD1 Products

Product Documents for Human/Mouse NeuroD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse NeuroD1 Antibody

For research use only

Citations for Human/Mouse NeuroD1 Antibody

Customer Reviews for Human/Mouse NeuroD1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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1 Stars
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  • Human/Mouse NeuroD1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: olfactory epithelium
    Species: Mouse
    Verified Customer | Posted 06/08/2016
    Human/Mouse NeuroD1 Antibody AF2746

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Protocols

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FAQs

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