Human/Mouse NeuroD1 Antibody

Catalog # Availability Size / Price Qty
NeuroD1 in  beta TC-6 Mouse Cell Line.
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Product Details
Citations (7)
Supplemental Products
Reviews (1)

Human/Mouse NeuroD1 Antibody Summary

Species Reactivity
Human, Mouse
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human NeuroD1
Accession # Q13562
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human NeuroD1
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NeuroD1 antibody in  beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

NeuroD1 in beta TC-6 Mouse Cell Line. Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name
Neurogenic Differentiation Factor 1
Entrez Gene IDs
4760 (Human)
Alternate Names
basic helix-loop-helix transcription factor; BETA2; BHF-1; BHLHA3; bHLHa3beta-cell E-box transactivator 2; Class A basic helix-loop-helix protein 3; MODY6; NeuroD; NeuroD1; neurogenic differentiation 1; neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; NIDDM

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Citations for Human/Mouse NeuroD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Progenitor cells from brown adipose tissue undergo neurogenic differentiation
    Authors: M Jumabay, L Zhang, J Yao, KI Boström
    Scientific Reports, 2022;12(1):5614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells
    Authors: H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be
    Nature Communications, 2021;12(1):3330.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats
    Authors: NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon
    Frontiers in Neuroscience, 2021;15(0):689601.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  5. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  6. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development
    Authors: N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton
    Cell Rep, 2019;27(8):2281-2291.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3.
    Authors: Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F
    FASEB J., 2007;21(9):2215-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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Human/Mouse NeuroD1 Antibody
By Anonymous on 06/08/2016
Application: ICC/IF Sample Tested: olfactory epithelium Species: Mouse