Human/Mouse NeuroD1 Antibody

Catalog # Availability Size / Price Qty
AF2746
AF2746-SP
NeuroD1 in  beta TC-6 Mouse Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse NeuroD1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NeuroD1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry NeuroD1 in  beta TC-6 Mouse Cell Line. View Larger

NeuroD1 in beta TC-6 Mouse Cell Line. Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name
Neurogenic Differentiation Factor 1
Entrez Gene IDs
4760 (Human)
Alternate Names
basic helix-loop-helix transcription factor; BETA2; BHF-1; BHLHA3; bHLHa3beta-cell E-box transactivator 2; Class A basic helix-loop-helix protein 3; MODY6; NeuroD; NeuroD1; neurogenic differentiation 1; neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; NIDDM

Product Datasheets

Citation for Human/Mouse NeuroD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3.
    Authors: Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F
    FASEB J., 2007;21(9):2215-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse NeuroD1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse NeuroD1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse NeuroD1 Antibody
By Anonymous on 06/08/2016
Application: ICC/IF Sample Tested: olfactory epithelium Species: Mouse