Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 240408
View all Oct-3/4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860

Specificity

Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Detection of Oct‑3/4 in NTERA-2 cells by Flow Cytometry

NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Oct‑3/4 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct‑3/4 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Oct‑3/4 antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line treated with 5 µM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References

  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Gene Symbol

POU5F1

UniProt

Q01860

Additional Oct-3/4 Products

Product Documents for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

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