Human/Mouse Oct-3/4 APC-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 240408
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Oct‑3/4
    Met1-Asn265 (Met262Leu)
    Accession # Q01860
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line treated with 5 μM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  • References:
    1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
    2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
    3. Rosner, M.H. et al. (1990) Nature 345:686.
    4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  • Long Name:
    Octamer-binding Protein 3/4
  • Entrez Gene IDs:
    5460 (Human); 18999 (Mouse)
  • Alternate Names:
    3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015;521(7552):316-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011;6(1):e16211.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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