Human/Mouse Oct-3/4 Antibody

(12 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 240408
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Oct‑3/4
    Met1-Asn265 (Met262Leu)
    Accession # Q01860
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    BG01V human embryonic stem cells and D3 mouse embryonic stem cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Oct-3/4 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry
Oct‑3/4 in BG01V Human Embryonic Stem Cells.

Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  • References:
    1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
    2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
    3. Rosner, M.H. et al. (1990) Nature 345:686.
    4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  • Long Name:
    Octamer-binding Protein 3/4
  • Entrez Gene IDs:
    5460 (Human); 18999 (Mouse)
  • Alternate Names:
    3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
    Authors: Hagiwara, Kunie, Obayashi, Takeshi, Sakayori, Nobuyuki, Yamanishi, Emiko, Hayashi, Ryuhei, Osumi, Noriko, Nakazawa, Toru, Nishida, Kohji
    PLoS ONE, 2014;9(1):e84072.
  3. Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis.
    Authors: Cortes-Dericks, Lourdes, Yazd, Ehsan Fa, Mowla, Seyed J, Schmid, Ralph A, Karoubi, Golnaz
    Anticancer Res, 2013;33(12):5365-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
    Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K
    Stem Cells Int, 2012;2012(0):875059.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
    Authors: Angel M, Yanik MF,
    PLoS ONE, 2010;5(7):e11756.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
    Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
    Cell Stem Cell, 2009;4(4):359-69.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
    Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
    Cancer Res., 2008;68(16):6533-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
    Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
    Stem Cells, 2008;26(10):2586-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 12 Citations
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