Human/Mouse Oct-3/4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1759P-100
IC1759P-025
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews

Human/Mouse Oct-3/4 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 240408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Oct-3/4 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Oct-3/4 antibody in Mouse Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1

Product Datasheets

You must select a language.

x

Citations for Human/Mouse Oct-3/4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
    Authors: F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot
    Sci Rep, 2019;9(1):8126.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
    Authors: M Dejosez, TP Zwaka
    Stem Cell Res, 2017;26(0):44-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
    PLoS ONE, 2016;11(9):e0161229.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
    Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
    Lab Invest, 2015;95(6):672-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Scalable passaging of adherent human pluripotent stem cells.
    Authors: Nie Y, Walsh P, Clarke D, Rowley J, Fellner T
    PLoS ONE, 2014;9(1):e88012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
    Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
    EMBO Mol Med, 2014;6(2):212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2012;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition.
    Authors: Nakahara M, Saeki K, Nakamura N
    Cloning Stem Cells, 2009;11(1):5-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
    Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
    Cancer Res., 2008;68(16):6533-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
    Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
    Stem Cells, 2007;25(6):1393-401.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse Oct-3/4 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse Oct-3/4 PE-conjugated Antibody and earn rewards!

Have you used Human/Mouse Oct-3/4 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review