Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody Summary
Met1-Trp444
Accession # AAC39602
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Orexin R2/HCRTR2 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Orexin R2/HCRTR2
Hypocretin Receptor 2 (HCRTR2), also known as Orexin Receptor 2 or OX2R, is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for Orexins A and B (Hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.
Product Datasheets
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