Human/Mouse PGC1 beta Antibody

Discontinued Product

AF5656 has been discontinued.
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Detection of Human PGC1 beta  by Western Blot.
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Product Details
Citations (1)
FAQs
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Human/Mouse PGC1 beta Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human/mouse PGC1 beta in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PGC1 beta
Ala315-Val420
Accession # Q86YN6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PGC1 beta  antibody by Western Blot. View Larger

Detection of Human PGC1 beta by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse PGC1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5656) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGC1 beta at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

PGC1 beta (peroxisome proliferator-activated receptor gamma coactivator 1 beta), also known as PPARGC1B, is an 110 kDa protein that belongs to a family of PPAR co‑activators. It coactivates nuclear receptors such as ERRalpha, upregulating expression of proteins that promote mitochondrial fusion and control basal mitochondrial biogenesis. N- and C-terminal alternate sequences, or deletion of amino acids (aa) 156‑194, produce isoforms of 984, 1017, 1023 (most common) and 1055 aa. Human PGC1 beta aa 315‑420, which is common to all isoforms, shares 79% and 76% aa identity with mouse and rat PGC1 beta, respectively.

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Citation for Human/Mouse PGC1 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Metabolic dysfunction consistent with premature aging results from deletion of Pim kinases.
    Authors: Din S, Konstandin M, Johnson B, Emathinger J, Volkers M, Toko H, Collins B, Ormachea L, Samse K, Kubli D, de la Torre A, Kraft A, Gustafsson A, Kelly D, Sussman M
    Circ Res, 2014-06-10;115(3):376-87.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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