Detection of Mouse Plexin A2 by Western Blot.
Western blot shows lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Plexin A2 in bEnd.3 Mouse Cell Line.
Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Plexin A2 in HUVEC Human Cells.
Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A2
Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily,
plexin family of proteins. It is found on cerebellar granule cells,
sensory neurons, and cardiac neural crest cells. It participates in cell
migration and axon guidance, and does so by serving as a receptor for
Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C.
Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane
glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636
aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508),
three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There
is one alternate start site 10 aa upstream of the standard start site.
Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with
human Plexin A2.
Entrez Gene IDs:
5362 (Human); 18845 (Mouse); 289392 (Rat)
FLJ11751; FLJ30634; KIAA0463plexin 2; OCT; OCTSemaphorin receptor OCT; Plexin A2; PLXN2; PLXN2plexin-A2; PLXNA2; transmembrane protein OCT
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