Human/Mouse/Rat APBA2 Antibody Summary
Accession # Q99767
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human APBA2 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
APBA2 (Adaptor protein X11 beta, also Mint2 and X11L) is a cytosolic member of the X11 family of adaptor proteins. Although its predicted MW is 83 kDa, it runs anomalously at 130-135 kDa in SDS-PAGE. APBA2 is expressed in neurons, and appears to play a role in A beta formation from APP. Normally, APBA2 associates with the cytoplasmic domain of transmembrane APP. This precludes its entry into lipid rafts, where it begins formation of A beta following cleaved by BACE. Human APBA2 is 749 amino acids (aa ) in length. It contains one phosphotyrosine binding (PTB) domain (aa 366-533) plus two PDZ domains (aa 568-734). Over aa 221-250, there is an APP interaction site that contains a key phosphorylation site at Ser238. There are two potential splice forms, one that shows a deletion of aa 406-417, and a second that contains a 44 aa substitution for aa 170-214. Over aa 1-165, human APBA2 shares 85% aa identity with mouse APBA2.
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