Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all APBA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APBA2
Ala2-Leu165
Accession # Q99767

Specificity

Detects human APBA2 in direct ELISAs and human, mouse and rat in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) APBA1 and rhAPBA3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat APBA2 Antibody

Detection of Human APBA2 antibody by Western Blot.

Detection of Human APBA2 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat APBA2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain (cortex) tissue and human brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APBA2

APBA2 (Adaptor protein X11 beta, also Mint2 and X11L) is a cytosolic member of the X11 family of adaptor proteins. Although its predicted MW is 83 kDa, it runs anomalously at 130-135 kDa in SDS-PAGE. APBA2 is expressed in neurons, and appears to play a role in A beta formation from APP. Normally, APBA2 associates with the cytoplasmic domain of transmembrane APP. This precludes its entry into lipid rafts, where it begins formation of A beta following cleaved by BACE. Human APBA2 is 749 amino acids (aa ) in length. It contains one phosphotyrosine binding (PTB) domain (aa 366-533) plus two PDZ domains (aa 568-734). Over aa 221-250, there is an APP interaction site that contains a key phosphorylation site at Ser238. There are two potential splice forms, one that shows a deletion of aa 406-417, and a second that contains a 44 aa substitution for aa 170-214. Over aa 1-165, human APBA2 shares 85% aa identity with mouse APBA2.

Long Name

Amyloid beta (A4) Precursor Protein-binding, Family A, Member 2

Alternate Names

LIN-10, MINT2, X11L

Entrez Gene IDs

321 (Human); 11784 (Mouse); 83610 (Rat)

Gene Symbol

APBA2

UniProt

Q99767 (Human)

Additional APBA2 Products

Product Documents for Human/Mouse/Rat APBA2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat APBA2 Antibody

For research use only

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