Human/Mouse/Rat ARHGAP1 Antibody Summary
Accession # Q07960
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat ARHGAP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat ARHGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARHGAP1 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ARHGAP1 (also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50 kDa member of the ARHGAP family of proteins. It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases. It does so by contributing an Arg residue (Arg282) to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules. Human ARHGAP1 is 439 amino acids (aa) in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa 53‑218), an SH3 binding site (aa 228‑238) and one C‑terminal RhoGAP domain (aa 244‑431). There are phosphorylation sites at Ser51 and Tyr65. There is one potential splice form with a 24 aa substitution for aa 1‑150 coupled to a 26 aa substitution for aa 300‑439. Over aa 1‑62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1.
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