Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Biliverdin Reductase B/BLVRB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Biliverdin Reductase B/BLVRB
Ala2-Gln206, predicted
Accession # P30043

Specificity

Detects human, mouse, and rat Biliverdin Reductase B/BLVRB in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB antibody by Western Blot.

Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB by Western Blot.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Biliverdin Reductase B/BLVRB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue

Reviewed Applications

Read 1 review rated 5 using AF6568 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Biliverdin Reductase B/BLVRB

Clearance of heme in mammals is a two-step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the  beta  isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance (1). BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues (2). The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and met‑hemoglobin (3, 4). BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha  isomer but could also use the  beta  isomer as substrate, BLVRB is specific for the beta  isomer (5, 6).

References

  1. Pereira, P.J. et al. (2001) Nat. Struct. Biol. 8:215.
  2. Chikuba, K. et al. (1994) Biochem. Biophys. Res. Commun. 198:1170.
  3. Shalloe, F. et al. (1996) Biochem. J. 316:385.
  4. Cunningham, O. et al. (2000) Biochem. J. 345:393.
  5. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
  6. Cunningham, O. et al. (2000) J. Biol. Chem. 275:19009.

Alternate Names

BLVRB, BVRB, FLR, GHBP

Entrez Gene IDs

645 (Human)

Gene Symbol

BLVRB

UniProt

P30043

Additional Biliverdin Reductase B/BLVRB Products

Product Documents for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

For research use only

Related Research Areas

Redox Enzymes

Citations for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

Customer Reviews for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody (1)

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  • Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell extract
    Species: Human
    Verified Customer | Posted 10/16/2020
    BLVRB was over-expressed in human megakaryocyte progenitor cells and BLVRB expression was examined by western blot. Molecular weights (kD) are indicated on the left.
    Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody AF6568

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