Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB by Western Blot.
Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Biliverdin Reductase B/BLVRB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Biliverdin Reductase B/BLVRB
Clearance of heme in mammals is a two-step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the beta isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance (1). BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues (2). The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and met‑hemoglobin (3, 4). BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha isomer but could also use the beta isomer as substrate, BLVRB is specific for the beta isomer (5, 6).
Pereira, P.J. et al. (2001) Nat. Struct. Biol. 8:215.
Chikuba, K. et al. (1994) Biochem. Biophys. Res. Commun. 198:1170.
Shalloe, F. et al. (1996) Biochem. J. 316:385.
Cunningham, O. et al. (2000) Biochem. J. 345:393.
Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
Cunningham, O. et al. (2000) J. Biol. Chem. 275:19009.
Entrez Gene IDs:
biliverdin reductase B (flavin reductase (NADPH)); Biliverdin Reductase B; Biliverdin-IX beta-reductase; BLVRB; BVRB; BVR-B; EC 18.104.22.168; EC 22.214.171.124; flavin reductase (NADPH); flavin reductase; FLR; FLRBVRB; FR; GHBP; Green heme-binding protein; MGC117413; NADPH-dependent diaphorase; NADPH-flavin reductase; SDR43U1; short chain dehydrogenase/reductase family 43U, member 1
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