Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

Catalog # Availability Size / Price Qty
AF6568
AF6568-SP
Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody in Western Blot
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Citations (1)
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Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Biliverdin Reductase B/BLVRB in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Biliverdin Reductase B/BLVRB
Ala2-Gln206, predicted
Accession # P30043
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB by Western Blot. View Larger

Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB by Western Blot.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Biliverdin Reductase B/BLVRB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Biliverdin Reductase B/BLVRB

Clearance of heme in mammals is a two-step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the  beta  isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance (1). BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues (2). The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and met‑hemoglobin (3, 4). BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha  isomer but could also use the  beta  isomer as substrate, BLVRB is specific for the beta  isomer (5, 6).

References
  1. Pereira, P.J. et al. (2001) Nat. Struct. Biol. 8:215.
  2. Chikuba, K. et al. (1994) Biochem. Biophys. Res. Commun. 198:1170.
  3. Shalloe, F. et al. (1996) Biochem. J. 316:385.
  4. Cunningham, O. et al. (2000) Biochem. J. 345:393.
  5. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
  6. Cunningham, O. et al. (2000) J. Biol. Chem. 275:19009.
Entrez Gene IDs
645 (Human)
Alternate Names
biliverdin reductase B (flavin reductase (NADPH)); Biliverdin Reductase B; Biliverdin-IX beta-reductase; BLVRB; BVRB; BVR-B; EC 1.3.1.24; EC 1.5.1.30; flavin reductase (NADPH); flavin reductase; FLR; FLRBVRB; FR; GHBP; Green heme-binding protein; MGC117413; NADPH-dependent diaphorase; NADPH-flavin reductase; SDR43U1; short chain dehydrogenase/reductase family 43U, member 1

Product Datasheets

Citation for Human/Mouse/Rat Biliverdin Reductase B/BLVRB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. In silico and crystallographic studies identify key structural features of biliverdin IX? reductase inhibitors having nanomolar potency
    Authors: NM Nesbitt, X Zheng, Z Li, JA Manso, WY Yen, LE Malone, J Ripoll-Roz, PJB Pereira, TJ Mantle, J Wang, WF Bahou
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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