Human/Mouse/Rat Cathepsin X/Z/P Antibody

Catalog # Availability Size / Price Qty
AF934
AF934-SP
Detection of Human, Mouse, and Rat Cathepsin X/Z/P by Western Blot.
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Product Details
Citations (6)
FAQs
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Human/Mouse/Rat Cathepsin X/Z/P Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Cathepsin X/Z/P in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Cathepsin C is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Cathepsin X/Z/P antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Cathepsin X/Z/P by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin X/Z/P at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin X/Z/P antibody in Human Breast and Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cathepsin X/Z/P in Human Breast and Breast Cancer Tissue. Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of normal human breast and breast cancer tissue using Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References
  1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
  2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
  3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
  4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
  5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
  6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, R. et al. (2001) Biol. Chem. 382:839.
  8. Therrien, C. et al. (2001) Biochemistry 40:2702.
  9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
Entrez Gene IDs
1522 (Human); 64138 (Mouse)
Alternate Names
Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSX; CTSZ

Product Datasheets

Citations for Human/Mouse/Rat Cathepsin X/Z/P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis
    Authors: Y Aiba, K Harada, M Ito, T Suematsu, S Aishima, Y Hitomi, N Nishida, M Kawashima, M Takatsuki, S Eguchi, S Shimoda, H Nakamura, A Komori, S Abiru, S Nagaoka, K Migita, H Yatsuhashi, K Tokunaga, M Nakamura
    Sci Rep, 2018;8(1):11808.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Cysteine cathepsins: Their biological and molecular significance in cancer stem cells
    Authors: P Anja, J Anahid, K Janko
    Semin. Cancer Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Cathepsin X Cleaves Profilin 1 C-Terminal Tyr139 and Influences Clathrin-Mediated Endocytosis.
    Authors: Pecar Fonovic U, Kos J
    PLoS ONE, 2015;10(9):e0137217.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix.
    Authors: Akkari L, Gocheva V, Kester J, Hunter K, Quick M, Sevenich L, Wang H, Peters C, Tang L, Klimstra D, Reinheckel T, Joyce J
    Genes Dev, 2014;28(19):2134-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Prognostic and predictive value of cathepsin X in serum from colorectal cancer patients.
    Authors: Vizin T, Christensen I, Wilhelmsen M, Nielsen H, Kos J
    BMC Cancer, 2014;14(0):259.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  6. Three-dimensional invasion of macrophages is mediated by cysteine cathepsins in protrusive podosomes.
    Authors: Jevnikar Z, Mirkovic B, Fonovic U, Zidar N, Svajger U, Kos J
    Eur J Immunol, 2012;42(12):3429-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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