Human/Mouse/Rat Cathepsin X/Z/P Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Cathepsin X/Z/P in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Cathepsin C is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
    Ala21-Val303 (Gly23Val, Ser48Thr)
    Accession # Q9UBR2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    3-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Cathepsin X/Z/P by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/
Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin X/Z/P at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Cathepsin X/Z/P in Human Breast and Breast Cancer Tissue. Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of normal human breast and breast cancer tissue using Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Cathepsin X/Z/P by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin X/Z/P at approximately 36 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

  • References:
    1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
    2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
    3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
    4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
    5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
    6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
    7. Menard, R. et al. (2001) Biol. Chem. 382:839.
    8. Therrien, C. et al. (2001) Biochemistry 40:2702.
    9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
    10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
    11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
  • Entrez Gene IDs:
    1522 (Human); 64138 (Mouse)
  • Alternate Names:
    Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSZ
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Cathepsin X Cleaves Profilin 1 C-Terminal Tyr139 and Influences Clathrin-Mediated Endocytosis.
    Authors: Pecar Fonovic U, Kos J
    PLoS ONE, 2015;10(9):e0137217.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Prognostic and predictive value of cathepsin X in serum from colorectal cancer patients.
    Authors: Vizin T, Christensen I, Wilhelmsen M, Nielsen H, Kos J
    BMC Cancer, 2014;14(0):259.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  3. Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix.
    Authors: Akkari L, Gocheva V, Kester J, Hunter K, Quick M, Sevenich L, Wang H, Peters C, Tang L, Klimstra D, Reinheckel T, Joyce J
    Genes Dev, 2014;28(19):2134-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Three-dimensional invasion of macrophages is mediated by cysteine cathepsins in protrusive podosomes.
    Authors: Jevnikar Z, Mirkovic B, Fonovic U, Zidar N, Svajger U, Kos J
    Eur J Immunol, 2012;42(12):3429-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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