to visualize the expression of Caveolin-1 by fluorescence microscopy for
staining cells and tissues. Conjugated Caveolin-1 antibodies are ideal for
immunocytochemistry colocalization studies in caveolae. Detects endogenous human, mouse and rat Caveolin-1 in Western blots.
Monoclonal Mouse IgG2B Clone # 7C8
Protein A or G purified from hybridoma culture supernatant
Puified rat adipocyte low density microsomes Accession # P41350
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Caveolin‑1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5736G) at 1:10 dilution overnight at 4° C and counterstained with Propidium Iodide (red). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.