Human/Mouse/Rat Caveolin-1 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Designed to visualize the expression of Caveolin-1 by fluorescence microscopy for staining cells and tissues. Conjugated Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. Detects endogenous human, mouse and rat Caveolin-1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 7C8
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Puified rat adipocyte low density microsomes
    Accession # P41350
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1:10 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

Caveolin‑1 in HeLa Human Cell Line. Caveolin‑1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5736G) at 1:10 dilution overnight at 4° C and counterstained with Propidium Iodide (red). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Caveolin-1
Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.
  • Entrez Gene IDs:
    857 (Human); 12389 (Mouse); 25404 (Rat)
  • Alternate Names:
    CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; Caveolin1; Caveolin-1; cell growth-inhibiting protein 32; MSTP085; VIP21
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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