Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7C8
View all Caveolin-1 Primary Antibodies »

Product Specifications

Immunogen

Puified rat adipocyte low density microsomes
Accession # P41350

Specificity

Detects endogenous human, mouse and rat Caveolin-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Caveolin‑1 Antibody

Detection of Human Caveolin‑1 antibody by Western Blot.

Detection of Human Caveolin‑1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caveolin-1 antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Caveolin‑1 in A431 Human Cell Line.

Caveolin-1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Caveolin‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells

Reviewed Applications

Read 2 reviews rated 5 using MAB5736 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Entrez Gene IDs

857 (Human); 12389 (Mouse); 25404 (Rat)

Gene Symbol

CAV1

UniProt

P41350

Additional Caveolin-1 Products

Product Documents for Human/Mouse/Rat Caveolin‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Caveolin‑1 Antibody

For research use only

Citations for Human/Mouse/Rat Caveolin‑1 Antibody

Customer Reviews for Human/Mouse/Rat Caveolin‑1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse/Rat Caveolin‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse/Rat Caveolin-1 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Thyroid tissue
    Species: Mouse
    Verified Customer | Posted 10/07/2021
    Human/Mouse/Rat Caveolin‑1 Antibody MAB5736
  • Human/Mouse/Rat Caveolin-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: abecular meshwork cells
    Species: Pig
    Verified Customer | Posted 11/28/2016
    Detection of caveolin1 in cultured pig trabecular meshwork cells using Human/Mouse/Rat Caveolin-1 Antibody (MAB5736) at 1:500 dilution overnight and donkey anti-mouse Alexa 488 (1:1000) for 1 hour.
    Human/Mouse/Rat Caveolin‑1 Antibody MAB5736

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies