Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse and rat Caveolin-1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 7C8
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Purified rat adipocyte low density microsomes
    Accession # P41350
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Caveolin-1 in HeLa Human Cell Line.

Caveolin-1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Biotinylated Monoclonal Antibody (Catalog # BAM5736) at 25 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 493-conjugated Streptavidin (green; Catalog # NL997) and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-1
Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (amino acids 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% amino acid sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.
  • Entrez Gene IDs:
    857 (Human); 12389 (Mouse); 25404 (Rat)
  • Alternate Names:
    CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; Caveolin1; Caveolin-1; cell growth-inhibiting protein 32; MSTP085; VIP21
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