CD2AP Antibody

R&D Systems | Catalog # AF4474

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD2AP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CD2AP
Ile423-Lys580
Accession # Q9Y5K6

Specificity

Detects endogenous human, mouse, and rat CD2AP in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for CD2AP Antibody

Detection of Human/Mouse/Rat CD2AP antibody by Western Blot.

Detection of Human/Mouse/Rat CD2AP by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for CD2AP Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, Y3-Ag rat myeloid cell line, KG-1 human acute myelogenous leukemia cell line, and M1 mouse myeloid leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD2AP

CD2AP (CD2-associated protein; also known as CMS) is an 80 kDa member of the CIN85/CD2AP family of adaptor proteins. Although CD2AP is ubiquitously expressed, it is often associated with glomerular podocytes. CD2AP serves as a scaffold between membrane proteins and the cytoskeleton. Through a Pro-rich region and SH3 domains, it is known to bind to such proteins as CD2, c-CBL, and p130Cas. Human CD2AP is 639 amino acids (aa) in length. It contains three SH3 homology domains (aa 4‑324), one Pro-rich region (aa 336‑422) and a C-terminal coiled-coil region that mediates homodimerization. Over aa 423‑580, human CD2AP shares 96% and 79% aa identity with dog and mouse CD2AP, respectively.

Long Name

CD2-associated protein

Alternate Names

CMS

Entrez Gene IDs

23607 (Human); 12488 (Mouse); 316258 (Rat)

Gene Symbol

CD2AP

UniProt

Q9Y5K6 (Human)

Additional CD2AP Products

Product Documents for CD2AP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for CD2AP Antibody

For research use only

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