Human/Mouse/Rat CD2AP Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat CD2AP by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human/Mouse/Rat CD2AP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse, and rat CD2AP in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human CD2AP
Accession # Q9Y5K6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat CD2AP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat CD2AP by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2AP

CD2AP (CD2-associated protein; also known as CMS) is an 80 kDa member of the CIN85/CD2AP family of adaptor proteins. Although CD2AP is ubiquitously expressed, it is often associated with glomerular podocytes. CD2AP serves as a scaffold between membrane proteins and the cytoskeleton. Through a Pro-rich region and SH3 domains, it is known to bind to such proteins as CD2, c-CBL, and p130Cas. Human CD2AP is 639 amino acids (aa) in length. It contains three SH3 homology domains (aa 4‑324), one Pro-rich region (aa 336‑422) and a C-terminal coiled-coil region that mediates homodimerization. Over aa 423‑580, human CD2AP shares 96% and 79% aa identity with dog and mouse CD2AP, respectively.

Long Name
CD2-associated protein
Entrez Gene IDs
23607 (Human); 12488 (Mouse); 316258 (Rat)
Alternate Names
adapter protein CMS; Cas ligand with multiple Src homology 3 (SH3) domains; CD2AP; CD2-associated protein; CMS; DKFZp586H0519

Product Datasheets

You must select a language.


Citations for Human/Mouse/Rat CD2AP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Visualisation studies and glomerular filtration in early diabetic rats
    Authors: Hiroshi Nakamoto
    J Biomech, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in human B cells.
    Authors: Niiro H, Jabbarzadeh-Tabrizi S, Kikushige Y, Shima T, Noda K, Ota S, Tsuzuki H, Inoue Y, Arinobu Y, Iwasaki H, Shimoda S, Baba E, Tsukamoto H, Horiuchi T, Taniyama T, Akashi K
    Blood, 2012;119(10):2263-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat CD2AP Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat CD2AP Antibody and earn rewards!

Have you used Human/Mouse/Rat CD2AP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review