Detection of Human, Mouse, and Rat CIB1 by Western Blot.
Western blot shows lysates of human spleen tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat CIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CIB1 (Calcium and Integrin-Binding protein 1; also calmyrin, KIP and CIBP) is a 22-23 kDa, Ca++-binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP3R, GPIIb/ alpha IIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP3R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the alpha IIb beta 3 integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191 amino acids (aa) in length. It contains a utilized myristoylation site at Gly2, followed by two EF-hand domains (aa 103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40 aa insertion after Lys29. Full-length human CIB1 (aa 1-191) shares 94% aa sequence identity with mouse CIB1.
Calcium and Integrin Binding 1
Entrez Gene IDs:
10519 (Human); 23991 (Mouse); 81823 (Rat)
calcium and integrin binding 1 (calmyrin); Calcium- and integrin-binding protein; Calmyrin; CIB1; CIBcalcium and integrin binding protein; CIBP; DNA-dependent protein kinase interacting protein; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; KIP1; KIPcalcium and integrin binding, protein kinase interacting protein; PRKDCIP; SIP2-28; SIP2-28calcium and integrin-binding protein 1; Snk interacting protein 2-28; SNK-interacting protein 2-28
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