Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody Summary
Accession # Q99962
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot. Western blot shows lysates of human brain stem tissue, human brain (hypothalamus) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin A1/SH3GL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin A1/SH3GL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endophilin A1/SH3GL2
Endophilin A1 (also SH3GL2, SH3P4, EEN-B1, CNS-A2, GL2 and SH3D2A) is a 39-42 kDa member of the A subfamily, endophilin (attracted to endocytic protein) family of molecules. It is a neuronally-expressed cytosolic protein that is concentrated in presynaptic areas. Endophilin A1 is a key player in clathrin-associated endocytosis, participating in invagination plus vesicle fission and recycling. It is able to impact membrane curvature through electroststic differences via its N‑terminus, and interact with multiple synaptic region proteins such as dynamin and synaptojanin through an SH3 homology domain at its C-terminus. Although Endophilin A1 is a monomer in the cytosol, it will homodimerize and heterodimerize with endophilin-2 when active at the synapse. Endophilin A1 is also associated with endocytic trafficking of growth factor receptors. In concert with CIN85, Cbl and ataxin-2, Endophilin A1 extend the half-life of EGFR at the plasma membrane. Human Endophilin A1 is 352 amino acids (aa) in length. It contains an aforementioned N-terminal BAR domain (aa 18-249) plus an SH3 domain that binds Pro‑rich aa sequences (aa 290-349). There is one potential alternative start site at Met36. Endophilin A1 is one of three groupA endophilin isoforms, all of which are the product of separate genes. Although the -A2 and -A3 isoforms share approximately 70% aa overall sequence identity with -A1, over aa 217-293, -A2 and -A3 share less than 50% aa sequence identity with Endophilin A1. Over this same aa sequence, human and mouse Endophilin A1 share more that 97% aa sequence identity.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody and earn rewards!
Have you used Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image