Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody

Catalog # Availability Size / Price Qty
AF8006
AF8006-SP
Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Endophilin A1/SH3GL2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Endophilin A1/SH3GL2
Ala217-Pro293
Accession # Q99962
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot. Western blot shows lysates of human brain stem tissue, human brain (hypothalamus) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin A1/SH3GL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin A1/SH3GL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endophilin A1/SH3GL2

Endophilin A1 (also SH3GL2, SH3P4, EEN-B1, CNS-A2, GL2 and SH3D2A) is a 39-42 kDa member of the A subfamily, endophilin (attracted to endocytic protein) family of molecules. It is a neuronally-expressed cytosolic protein that is concentrated in presynaptic areas. Endophilin A1 is a key player in clathrin-associated endocytosis, participating in invagination plus vesicle fission and recycling. It is able to impact membrane curvature through electroststic differences via its N‑terminus, and interact with multiple synaptic region proteins such as dynamin and synaptojanin through an SH3 homology domain at its C-terminus. Although Endophilin A1 is a monomer in the cytosol, it will homodimerize and heterodimerize with endophilin-2 when active at the synapse. Endophilin A1 is also associated with endocytic trafficking of growth factor receptors. In concert with CIN85, Cbl and ataxin-2, Endophilin A1 extend the half-life of EGFR at the plasma membrane. Human Endophilin A1 is 352 amino acids (aa) in length. It contains an aforementioned N-terminal BAR domain (aa 18-249) plus an SH3 domain that binds Pro‑rich aa sequences (aa 290-349). There is one potential alternative start site at Met36. Endophilin A1 is one of three groupA endophilin isoforms, all of which are the product of separate genes. Although the -A2 and -A3 isoforms share approximately 70% aa overall sequence identity with -A1, over aa 217-293, -A2 and -A3 share less than 50% aa sequence identity with Endophilin A1. Over this same aa sequence, human and mouse Endophilin A1 share more that 97% aa sequence identity.

Long Name
SH3-domain GRB2-like 2
Entrez Gene IDs
6456 (Human); 20404 (Mouse); 116743 (Rat)
Alternate Names
CNSA2; CNSA2FLJ25015; EEN-B1; EEN-B1bA335L15.1 (SH3-domain GRB2-like 2); Endophilin A1; Endophilin-1; endophilin-A1; FLJ20276; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3D2AEndophilin A1 BAR domain; SH3-domain GRB2-like 2; SH3GL2; SH3P4; SH3P4endophilin-1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody and earn rewards!

Have you used Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review