Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Endophilin A1/SH3GL2 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Endophilin A1/SH3GL2
    Ala217-Pro293
    Accession # Q99962
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot. Western blot shows lysates of human brain stem tissue, human brain (hypothalamus) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin A1/SH3GL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin A1/SH3GL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endophilin A1/SH3GL2

Endophilin A1 (also SH3GL2, SH3P4, EEN-B1, CNS-A2, GL2 and SH3D2A) is a 39-42 kDa member of the A subfamily, endophilin (attracted to endocytic protein) family of molecules. It is a neuronally-expressed cytosolic protein that is concentrated in presynaptic areas. Endophilin A1 is a key player in clathrin-associated endocytosis, participating in invagination plus vesicle fission and recycling. It is able to impact membrane curvature through electroststic differences via its N‑terminus, and interact with multiple synaptic region proteins such as dynamin and synaptojanin through an SH3 homology domain at its C-terminus. Although Endophilin A1 is a monomer in the cytosol, it will homodimerize and heterodimerize with endophilin-2 when active at the synapse. Endophilin A1 is also associated with endocytic trafficking of growth factor receptors. In concert with CIN85, Cbl and ataxin-2, Endophilin A1 extend the half-life of EGFR at the plasma membrane. Human Endophilin A1 is 352 amino acids (aa) in length. It contains an aforementioned N-terminal BAR domain (aa 18-249) plus an SH3 domain that binds Pro‑rich aa sequences (aa 290-349). There is one potential alternative start site at Met36. Endophilin A1 is one of three groupA endophilin isoforms, all of which are the product of separate genes. Although the -A2 and -A3 isoforms share approximately 70% aa overall sequence identity with -A1, over aa 217-293, -A2 and -A3 share less than 50% aa sequence identity with Endophilin A1. Over this same aa sequence, human and mouse Endophilin A1 share more that 97% aa sequence identity.

  • Long Name:
    SH3-domain GRB2-like 2
  • Entrez Gene IDs:
    6456 (Human); 20404 (Mouse); 116743 (Rat)
  • Alternate Names:
    CNSA2; CNSA2FLJ25015; EEN-B1; EEN-B1bA335L15.1 (SH3-domain GRB2-like 2); Endophilin A1; Endophilin-1; endophilin-A1; FLJ20276; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3D2AEndophilin A1 BAR domain; SH3-domain GRB2-like 2; SH3GL2; SH3P4; SH3P4endophilin-1
Related Research Areas
Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

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Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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