Human/Mouse/Rat FoxP3 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FoxP3
    Gln105-Lys200
    Accession # Q9BZS1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Mouse Anti-Human CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB3791C) or (B) Mouse Anti-Human IL‑2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).
Intracellular Staining by Flow Cytometry
Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Rat Anti-Mouse CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat Anti-Mouse IL‑2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP3
Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.
  • Long Name:
    Forkhead Box P3
  • Entrez Gene IDs:
    50943 (Human); 20371 (Mouse)
  • Alternate Names:
    AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A small-molecule inhibitor of macrophage migration inhibitory factor for the treatment of inflammatory disease.
    Authors: Kithcart AP, Cox GM, Sielecki T
    FASEB J., 2010;24(11):4459-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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